Prohibitin (PHB) antibody
| Antigen |
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| Synonyms |
PHB1, Bap32, sr:nyz128, wu:fa18f07, wu:fb09f05, phb1, MGC53103, MGC75944, PHB, MGC127238, Phb, ACYPI000080, phb |
| Clonality |
Monoclonal (II-14-10) |
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Host
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Reactivity
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Human (72), Rat (Rattus) (52), Mouse (Murine) (40), Dog (Canine) (20), Horse (Equine) (18), Chicken (3), Cow (Bovine) (3), Monkey (2), Pig (Porcine) (2), Cat (Feline) (1), Rabbit (1), Xenopus laevis (1), Zebrafish (1)
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Conjugate
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Biotin (7), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB) (73), ELISA (38), Immunofluorescence (IF) (37), Immunohistochemistry (IHC) (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (28), Immunoprecipitation (IP) (19), Flow Cytometry (FACS) (8), Immunocytochemistry (ICC) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
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| Catalog no. |
ABIN105895 |
| Quantity |
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| Price |
598.40 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 3 to 4 Business Days |
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Alternative name
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Prohibitin
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Immunogen
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BALB/C mice were injected with purified recombinant rat prohibitin protein.
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Format
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Ig-fraction
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Isotype
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IgG1
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Clone
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II-14-10
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Specificity
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This antibody is specific to 30 kD protein known as Prohibitin. Prohibitin is an evolutionarily conserved protein located in the inner membrane of mitochondria. Prohibitin shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization and tumor suppression. This antibody cross reacts with mouse, rat and pig.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Heart. Cellular localization: Cytoplasmic Other Applications: Western Blotting, Immunoprecipitation, Immunofluorescence
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Concentration
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100ug/ml approximately
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Purification
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Ig-fraction
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Buffer
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This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Sato, Saito, Swensen et al.: "The human prohibitin gene located on chromosome 17q21 is mutated in sporadic breast cancer." in: Cancer research, Vol. 52, Issue 6, pp. 1643-6, 1992 (PubMed).
Terashima, Kim, Adachi et al.: "The IgM antigen receptor of B lymphocytes is associated with prohibitin and a prohibitin-related protein." in: The EMBO journal, Vol. 13, Issue 16, pp. 3782-92, 1994 (PubMed).
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Alternatives for antigen "Prohibitin (PHB)", type "Antibodies"
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Hosts
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Rabbit (65), Mouse (29)
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Reactivities
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Human (72), Rat (Rattus) (52), Mouse (Murine) (40), Dog (Canine) (20), Horse (Equine) (18), Chicken (3), Cow (Bovine) (3), Monkey (2), Pig (Porcine) (2), Cat (Feline) (1), Rabbit (1), Xenopus laevis (1), Zebrafish (1)
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Applications
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Western Blotting (WB) (73), ELISA (38), Immunofluorescence (IF) (37), Immunohistochemistry (IHC) (28), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (28), Immunoprecipitation (IP) (19), Flow Cytometry (FACS) (8), Immunocytochemistry (ICC) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
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Conjugates
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Biotin (7), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Epitopes
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C-Term (28), N-Term (4), Center (2), AA 100-150 (1), AA 50-100 (1), C-Term,Glu194 (1), Internal Region (1)
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