Proliferating Cell Nuclear Antigen (PCNA) antibody

Antigen: Proliferating Cell Nuclear Antigen (PCNA)

Synonyms: MGC8367, PCNAR, Pcna/cyclin, pcna

Clonality: Monoclonal (PC10)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN105947

Additional Information

Immunogen: Rat PCNA made in the protein A expression vector pR1T2T.

Format: Supernatant

Isotype: IgG2a kappa

Clone: PC10

Specificity: PCNA is essential for cellular DNA synthesis. This antibody reacts with proliferating cells in a wide range of normal tissues. The significance of PCNA immunoreactivity in tumors in not fully established yet. However, in non-Hodgkin's lymphoma a linear relation between Ki-67 and PCNA staining has been demonstrated.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Proliferation Markers

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Waseem, Lane: "Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form." in: Journal of cell science, Vol. 96 ( Pt 1), pp. 121-9, 1990 (PubMed).

Hall, Levison, Woods et al.: "Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms." in: The Journal of pathology, Vol. 162, Issue 4, pp. 285-94, 1991 (PubMed).

Takasaki, Fishwild, Tan: "Characterization of proliferating cell nuclear antigen recognized by autoantibodies in lupus sera." in: The Journal of experimental medicine, Vol. 159, Issue 4, pp. 981-92, 1984 (PubMed).