PCNA antibody (Biotin)
| Antigen |
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| Clonality |
Monoclonal (PC10) |
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Host
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Reactivity
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 Alternatives Mouse (Murine), Human, Rat (Rattus), Vertebrates, Insect
Human (141), Rat (Rattus) (94), Mouse (Murine) (70), Cow (Bovine) (39), Dog (Canine) (39), Rabbit (36), Chicken (19), Horse (Equine) (6), Pig (Porcine) (5), Fish (4), Vertebrates (4), Yeast (Pichia pastoris) (4), Amphibian (3), Fruit Fly (Drosophila melanogaster) (3), Insect (3), Mammalian (3), Yeast (Saccharomyces Cerevisiae) (3), Hamster (1), Monkey (1), Primate (1), Sheep (Ovine) (1), Xenopus laevis (1)
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Conjugate
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FITC (14), Biotin (9), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), PE (4), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2), APC (1), RPE (1)
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Application
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Western Blotting (WB) (97), Flow Cytometry (FACS) (68), Immunofluorescence (IF) (62), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (60), Immunoprecipitation (IP) (31), ELISA (19), Enzyme Immunoassay (EIA) (15), Immunohistochemistry (IHC) (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), Immunocytochemistry (ICC) (5), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1)
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| Catalog no. |
ABIN105950 |
| Quantity |
0.2 mg (0.1 mg/ml) |
| Price |
272.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 10 Business Days |
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Immunogen
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Rat PCNA made in the protein A vector pR1T2T
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Isotype
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IgG2a
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Clone
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PC10
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Specificity
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Anti-PCNA (Proliferating Cell Nuclear Antigen) recognizes polymerase delta accessory protein (Mr 36 kDa).1,3 This auxiliary protein is also known as cyclin and experiments strongly suggest that PCNA is essential for cellular DNA synthesis.2,3 Antigen Distribution: Anti-PCNA staining is almost entirely confined to the nucleus and may show a diffuse or granular pattern or a mixture of both. Dominant PCNA expression begins during late G1 phase and is present through S phase of the cell cycle.
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Application Notes
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FC. Each Investigator should determine their own optimal working dilution for specific applications.
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Concentration
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0.1 mg/ml
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Buffer
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This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
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Storage
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This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
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Research Area
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Proliferation Markers
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Restrictions
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For Research Use only
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Alternatives for antigen "PCNA", type "Antibodies"
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Hosts
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Mouse (98), Rabbit (78)
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Reactivities
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Human (141), Rat (Rattus) (94), Mouse (Murine) (70), Cow (Bovine) (39), Dog (Canine) (39), Rabbit (36), Chicken (19), Horse (Equine) (6), Pig (Porcine) (5), Fish (4), Vertebrates (4), Yeast (Pichia pastoris) (4), Amphibian (3), Fruit Fly (Drosophila melanogaster) (3), Insect (3), Mammalian (3), Yeast (Saccharomyces Cerevisiae) (3), Hamster (1), Monkey (1), Primate (1), Sheep (Ovine) (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (97), Flow Cytometry (FACS) (68), Immunofluorescence (IF) (62), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (60), Immunoprecipitation (IP) (31), ELISA (19), Enzyme Immunoassay (EIA) (15), Immunohistochemistry (IHC) (13), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), Immunocytochemistry (ICC) (5), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1)
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Conjugates
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FITC (14), Biotin (9), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (5), PE (4), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2), APC (1), RPE (1)
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