Prostate Specific Antigen (PSA) antibody

Antigen: Prostate Specific Antigen (PSA)

Clonality: Monoclonal (ER-PR8)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN106012

Additional Information

Immunogen: BALB/C mice were immunized with purified human PSA.

Format: Supernatant

Isotype: IgG1-kappa

Clone: ER-PR8

Specificity: This antibody reacts with human prostate specific antigen. It stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Prostate carcinoma. Cellular localization: Cytoplasm Other Applications: Western Blotting (1:50 to 1:100)

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell/Tissue Markers, Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human prostate carcinoma stained with PSA antibody

Publications

Publications: Gallee, van Vroonhoven, van der Korput et al.: "Characterization of monoclonal antibodies raised against the prostatic cancer cell line PC-82." in: The Prostate, Vol. 9, Issue 1, pp. 33-45, 1986 (PubMed).