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Antibodies
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anti-Prostate Stem Cell Antigen (PSCA) antibody
Prostate Stem Cell Antigen (PSCA) antibody
| Antigen |
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| Synonyms |
PRO232, MGC123434, 2210408B04Rik, PSCA |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Western Blotting (WB) (14), ELISA (7), Enzyme Immunoassay (EIA) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN106022 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
526.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Immunogen
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A synthetic peptide derived from extracellular region of human PSCA protein.
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with a ~14 kD protein. Prostate Stem Cell Antigen (PSCA) is a cell surface antigen which is overexpressed in ~ 40% of primary prostate cancers and in nearly 100% of metastatic ones. PSCA is also overexpressed in a majority of transitional cell and pancreatic carcinomas. Antibody directed against PSCA inhibits tumorigenesis, slows tumor growth, prolongs survival and prevents metastasis in a preclinical zenograft model.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Bladder carcinoma, prostate carcinoma. Cellular localization: Cell membrane, cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against PSCA containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cell/Tissue Markers, Cancer
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Prostate Stem Cell Antigen (PSCA)", type "Antibodies"
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Hosts
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Rabbit (39), Mouse (6), Goat (2)
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Reactivities
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Human (45), Dog (Canine) (2), Mouse (Murine) (2), Cow (Bovine) (1)
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Applications
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Western Blotting (WB) (14), ELISA (7), Enzyme Immunoassay (EIA) (7), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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Center (2)
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