Protein A antibody

Antigen: Protein A

Protein Type: Native

Clonality: Polyclonal

Host: Goat

Reactivity: Staphylococcus aureus (S. aureus)

Conjugate: Un-conjugated

Application: Please enquire

Catalog no.: ABIN106169

Additional Information

Immunogen: Protein A [Staphylococcus aureus]

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Isotype: IgG

Description: Anti-Protein A Antibody detects Protein A. Protein A is a 56 kDa surface protein originally found in the cell wall of the bacterium Staphylococcus aureus encoded by the spa gene. Protein A is an efficient binding protein to the immunoglobulins and is used in biochemical research because of its ability to bind .Protein A binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. It does not react with human IgG3 or IgD, nor will it react to mouse IgM, IgA or IgE. Protein A is commonly used in bioprocessing and bioseparations.

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Application Details

Application Notes: Protein A Antibody is suitable for immunoprecipitation, immunodiffusion, conjugation and most immunological methods requiring high titer and specificity. Opitimal concentriaton in immunoassays should be determined by the researcher.
ELISA Dilution: 1:20,000 - 1:100,000
Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Western Blot Dilution: 1:2,000 - 1:10,000

Concentration: 10.0 mg/mL

Purity: This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium azide

Storage: Expiration date is one (1) year from date of opening. None. Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. 

Research Area: Coagulation

Restrictions: For Research Use only

Images

Western Blot of Goat anti-Protein A antibody. Lane 1: 1.0 ug of protein reduced. Lane 2: 0.25 µg of protein reduced. Lane 3: 1.0 ug of protein non-reduced. Lane 4: 0.25 µg of protein non-reduced. Load: 35 µg per lane. Primary antibody: anti Protein A antibody at 1:5000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: MB-070 overnight at 4°C.

Publications

Ramirez, Loch, Zhang et al.: "Use of a single-chain antibody library for ovarian cancer biomarker discovery." in: Molecular & cellular proteomics : MCP, Vol. 9, Issue 7, pp. 1449-60, 2010 (PubMed).