Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1) antibody

Antigen: Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1)

Synonyms: cb358, wu:fc55h08, UCHL1, UCH-L1, MGC133697, PARK5, PGP95, PGP9.5, Uch-L1, park5, pgp9.5, uch-l1, MGC132191, uchl1, gad, C88048, R75593, AW822034

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine)

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN106227

Additional Information

Alternative name: Protein Gene Product 9.5 (PGP 9.5)

Immunogen: Purified PGP 9.5 protein isolated from bovine brain.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 25-27 kD protein. Protein gene protein 9.5 is a ubiquitin C-terminal hydroxylase found in neurons and nerve fibers of central and peripheral nervous system, many neuroendocrinal cells, segments of renal tubules, spermatogonia, leydig cells of testis and ova. It is present in cellular inclusions and can be a useful neuronal marker and in the studies of neurodegenerative disorders. This antibody cross reacts with cow.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreatic tumor. Cellular localization: Nuclear and cytoplasmic Other Applications: Immunofluorescence

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against bovine PGP 9.5 containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Rode, Dhillon, Doran et al.: "PGP 9.5, a new marker for human neuroendocrine tumours." in: Histopathology, Vol. 9, Issue 2, pp. 147-58, 1985 (PubMed).

Vered, Carpenter, Buchner: "Granular cell tumor of the oral cavity: updated immunohistochemical profile." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 38, Issue 1, pp. 150-9, 2009 (PubMed).

Vered, Dobriyan, Buchner: "Congenital granular cell epulis presents an immunohistochemical profile that distinguishes it from the granular cell tumor of the adult." in: Virchows Archiv : an international journal of pathology, Vol. 454, Issue 3, pp. 303-10, 2009 (PubMed).