Phosphatase and Tensin Homolog (PTEN) antibody

Antigen: Phosphatase and Tensin Homolog (PTEN)

Synonyms: BZS, MHAM, TEP1, MMAC1, PTEN1, 10q23del, MGC11227, AI463227, MGC183880, 2310035O07Rik, A130070J02Rik, B430203M17Rik, Mmac, PTEN, bzs, mham, tep1, mmac1, pten1, Pten

Clonality: Monoclonal (28H6)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN106347

Additional Information

Alternative name: PTEN

Immunogen: BALB/C mice were injected with prokaryotic recombinant protein corresponding to a 200 amino acid C-terminal region of the PTEN molecule.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 28H6

Specificity: This antibody reacts with human PTEN also known as MMAC or TEP1. PTEN is a tumor suppressor gene, which encodes a multifunctional phosphatase, which is expressed almost ubiquitously and regulates the cell cycle, apoptosis and cell adhesion. Deletions and mutations of PTEN occur in a range of cancers including breast cancer, malignant melanoma, endometrial carcinoma, bladder carcinoma, small cell lung carcinoma and endometrial ovarian carcinoma.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 to 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Signaling, Transcription Factors

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Lu, Lin, LaPushin et al.: "The PTEN/MMAC1/TEP tumor suppressor gene decreases cell growth and induces apoptosis and anoikis in breast cancer cells." in: Oncogene, Vol. 18, Issue 50, pp. 7034-45, 2000 (PubMed).

Paramio, Navarro, Segrelles et al.: "PTEN tumour suppressor is linked to the cell cycle control through the retinoblastoma protein." in: Oncogene, Vol. 18, Issue 52, pp. 7462-8, 2000 (PubMed).

Mutter, Lin, Fitzgerald et al.: "Altered PTEN expression as a diagnostic marker for the earliest endometrial precancers." in: Journal of the National Cancer Institute, Vol. 92, Issue 11, pp. 924-30, 2000 (PubMed).