Quinolinic Acid antibody
| Antigen |
|
| Clonality |
Polyclonal |
|
Host
|
|
|
Application
|
|
| Catalog no. |
ABIN106395 |
| Quantity |
100 µl |
| Price |
931.36 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Immunogen
|
Synthetic Quinolinic acid conjugated to bovine serum albumin (BSA) Specificity Using a conjugate Quinolinic acid-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio(a) Quinolinic acid-BSA 1 Kynurenic-BSA 1/50,000 Phenylalanine-BSA 1/50,000 (a): Quinolinic acid-BSA concentration/Other conjugated close related compounds concentration at half displacement. BSA: Bovine Serum Albumin.
|
|
Format
|
Lyophilized
|
|
Isotype
|
IgG (Matching secondary antibodies)
|
|
Specificity
|
Conjugated Quinolinic acid .
|
|
Application Notes
|
Example of Immunohistochemistry protocol Perfusion protocol for Adult male Sprague Dawley (weight around 0.5 kg):1-The animals can be deeply anaesthetized (for example with urethane-0.5-1.5g/kg, intraperitoneal). 2-Heparinized, and perfused via the ascending aorta with 50 ml of MES (2-Morpholinoethanesulfonic acid monohydrate Fluka) 10 -1 Recommended dilution for Immunohistochemistry (1/1,000-1/5,000) Recommended dilution for Western blot (1/1,000-1/12,000)
|
|
Purity
|
Antiserum previously preabsobed on protein carriers, and purified.
|
|
Storage
|
Lyophilized antibodies are stable at least 2 years. After reconstitution with 50µl of distilled water and 50µl of glycerol, the aliquot can be repeated freezed (up to five times).
|
|
Restrictions
|
For Research Use only
|
|
Publications
|
Guillemin, Brew, Noonan et al.: "Indoleamine 2,3 dioxygenase and quinolinic acid immunoreactivity in Alzheimer's disease hippocampus." in: Neuropathology and applied neurobiology, Vol. 31, Issue 4, pp. 395-404, 2005 (PubMed).
Guillemin, Cullen, Lim et al.: "Characterization of the kynurenine pathway in human neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 27, Issue 47, pp. 12884-92, 2007 (PubMed).
|
Alternatives for antigen "Quinolinic Acid", type "Antibodies"
Alternatives 
