RAS antibody

Antigen: RAS

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN106439

Additional Information

Immunogen: A synthetic peptide corresponding to amino acid 31 43 (EYDPTIEDSYRKQ) from L2 loop of human Ras protein.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 21kD protein. Each mammalian cell contains three Ras proto-oncogene coding for closely related Ras proteins: H-, K- and N-Ras. Oncogenic mutations in Ras genes are present in ~30% of all human cancers. K-Ras mutations are common in pancreatic, colorectal and non-small-cell lung carcinomas, H-Ras mutations are common in bladder, kidney and thyroid carcinomas, N-Ras mutations are found in melanoma, hematological malignancies and hepatocellular carcinoma. This antibody stain H-, K- and N-Ras proteins. This antibody cross reacts with mouse, rat and cow.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cytoplasmic, nuclear

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against Ras containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with Ras antibody