RET antibody

Antigen: RET

Clonality: Monoclonal (RET01)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN106529

Additional Information

Immunogen: BALB/C mice were injected with recombinant protein encoding the extreme C-terminal cytoplasmic region of the human ret oncoprotein.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: RET01

Specificity: Ret proto-oncogene encodes a cell surface glycoprotein belonging to a member of the receptor tyrosine kinase family and is located on chromosome 10q11.2. Ret is observed in several regions of the central nervous system, in the developing cranial nerve ganglia and a subset of cells within dorsal root ganglia, in motor neurons in the spinal cord and hindbrain.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:20 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 90 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Small intestine. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Signaling, Kinases/Phosphatases

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human small intestine stained with ret oncoprotein antibody

Publications

Publications: Nakamura, Ishizaka, Nagao et al.: "Expression of the ret proto-oncogene product in human normal and neoplastic tissues of neural crest origin." in: The Journal of pathology, Vol. 172, Issue 3, pp. 255-60, 1994 (PubMed).

Williams, Rooney, Carss et al.: "Analysis of the RET proto-oncogene in sporadic parathyroid adenomas." in: The Journal of pathology, Vol. 180, Issue 2, pp. 138-41, 1997 (PubMed).

Nosrat, Tomac, Hoffer et al.: "Cellular and developmental patterns of expression of Ret and glial cell line-derived neurotrophic factor receptor alpha mRNAs." in: Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale, Vol. 115, Issue 3, pp. 410-22, 1997 (PubMed).