Replication Protein A2, 32kDa (RPA2) antibody

Antigen: Replication Protein A2, 32kDa (RPA2)

Synonyms: REPA2, RPA32, 153194_at, dRPA2, DmelCG9273, CG9273, rpa, zgc:109822, ik:tdsubc_2g1, xx:tdsubc_2g1, RPA2, repa2, rpa32, MGC79561, MGC137236, Rpa2, GB13976, DKFZp468O0923, LOC100230445

Clonality: Monoclonal (9H8)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN106591

Additional Information

Alternative name: RPA/p34

Immunogen: BALB/C mice were injected with purified human RPA protein.

Format: Ig-fraction

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: 9H8

Specificity: This antibody reacts with a 32-34 kD protein known as Replication Protein A (RPA). RPA is involved in DNA replication, repair and recombination. Human RPA is a stable heterotrimer of 70 kD, 32-34 kD and 11-14 kD subunits (RPA70, RPA32 and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding for SV40 DNA and stimulates DNA polymerase alpha and delta.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Nuclear Other Applications: Immunoprecipitation, Immunofluorescence, Western Blotting

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: DNA/RNA

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human tonsil stained with RPA/p34 antibody

Publications

Publications: Pan, Park, Amin et al.: "Phosphorylated and unphosphorylated forms of human single-stranded DNA-binding protein are equally active in simian virus 40 DNA replication and in nucleotide excision repair." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 10, pp. 4636-40, 1995 (PubMed).