S-100 Protein (alpha chain) antibody

Antigen: S-100 Protein (alpha chain)

Clonality: Monoclonal (SH-B1)

Host: Mouse

Reactivity: Human, Rat (Rattus), Rabbit, Cat (Feline), Pig (Porcine)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN106614

Additional Information

Alternative name: S-100 Protein

Immunogen: Purified bovine brain S-100b preparation.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: SH-B1

Specificity: This antibody is specific against an epitope located on the beta-chain (i.e. in S-100a and S-100b) but not on the alpha-chain of S- 100 (i.e. in S-100a and S-100ao). This antibody cross reacts with human, porcine, rabbit, cat and rat tissues. This antibody can be used to localize S-100a and S-100b in various tissue sections.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Proteolytic digestion of tissue sections is required prior to immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Kan-Mitchell, Rao, Albert et al.: "S100 immunophenotypes of uveal melanomas." in: Investigative ophthalmology & visual science, Vol. 31, Issue 8, pp. 1492-6, 1990 (PubMed).

Baudier, Glasser, Gerard: "Ions binding to S100 proteins. I. Calcium- and zinc-binding properties of bovine brain S100 alpha alpha, S100a (alpha beta), and S100b (beta beta) protein: Zn2+ regulates Ca2+ binding on S100b protein." in: The Journal of biological chemistry, Vol. 261, Issue 18, pp. 8192-203, 1986 (PubMed).

Takahashi, Isobe, Ohtsuki et al.: "Immunohistochemical study on the distribution of alpha and beta subunits of S-100 protein in human neoplasm and normal tissues." in: Virchows Archiv. B, Cell pathology including molecular pathology, Vol. 45, Issue 4, pp. 385-96, 1984 (PubMed).

Mani, Boyes, Kay: "Physicochemical and optical studies on calcium- and potassium-induced conformational changes in bovine brain S-100b protein." in: Biochemistry, Vol. 21, Issue 11, pp. 2607-12, 1982 (PubMed).