S-100 Protein (alpha chain) antibody

Antigen: S-100 Protein (alpha chain)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN106623

Additional Information

Alternative name: S-100 Protein

Immunogen: Affinity purified S-100 protein isolated from cow brain.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with cow S-100 protein A and B. It shows strong cross-reactivity with human S-100 A and B. In the brain, it labels glial and ependymal cells. Melanocytes and Langerhans cells of the skin stain positive with this antibody.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:200 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Melanoma. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against S-100 containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cell Cycle

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Winek, Scheithauer, Wick: "Meningioma, meningeal hemangiopericytoma (angioblastic meningioma), peripheral hemangiopericytoma, and acoustic schwannoma. A comparative immunohistochemical study." in: The American journal of surgical pathology, Vol. 13, Issue 4, pp. 251-61, 1989 (PubMed).

Moore: "A soluble protein characteristic of the nervous system." in: Biochemical and biophysical research communications, Vol. 19, Issue 6, pp. 739-44 (PubMed).

Kindblom, Lodding, Rosengren et al.: "S-100 protein in melanocytic tumors. An immunohistochemical investigation of benign and malignant melanocytic tumors and metastases of malignant melanoma and a characterization of the antigen in comparison to human brain." in: Acta pathologica, microbiologica, et immunologica Scandinavica. Section A, Pathology, Vol. 92, Issue 4, pp. 219-30, 1984 (PubMed).