Baculoviral IAP Repeat-Containing 5 (BIRC5) antibody

Antigen: Baculoviral IAP Repeat-Containing 5 (BIRC5)

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN107168

Additional Information

Alternative name: Survivin

Immunogen: A synthetic peptide derived from the C-terminal region of Survivin.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 16.5 kD protein, known as Survivin. Survivin is an inhibitor of apoptosis protein. It is expressed in the G2/M phase of the cell cycle in a cycle-regulated manner. At the beginning of mitosis, survivin associates with microtubules of the mitotic spindle. Disruption of survivin-microtubule interactions results in loss of survivins antiapoptosis function and increased Caspase-3 activity. This antibody cross reacts with human and mouse.

Synonyms: API4, EPR-1, AP14, BIRC5, MGC133855, DKFZp468N162, Api4, TIAP, AAC-11, survivin40, bir5a, survivin, bir1-A, svv1, XBIR1, Xsvv1, birc5.1-A, xSurvivin, XSurvivin1, LOC398389, LOC398418, Su2, birc5.1-B, BIR1, Survivin, Survivin1, xSurvivin1, SIX, Su1, api4, svv2, birc5, epr-1, xSurvivin2

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:250-1:500 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. High temperature treatment of formalin-fixed tissue sections with 10mM citrate buffer, pH 6.0 must be performed prior to the immunostaining. Positive Control: Stomach. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human Survivin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer, Apoptosis/Necrosis, Neurology

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Ambrosini, Adida, Altieri: "A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma." in: Nature medicine, Vol. 3, Issue 8, pp. 917-21, 1997 (PubMed).

Lu, Altieri, Tanigawa: "Expression of a novel antiapoptosis gene, survivin, correlated with tumor cell apoptosis and p53 accumulation in gastric carcinomas." in: Cancer research, Vol. 58, Issue 9, pp. 1808-12, 1998 (PubMed).

Li, Ambrosini, Chu et al.: "Control of apoptosis and mitotic spindle checkpoint by survivin." in: Nature, Vol. 396, Issue 6711, pp. 580-4, 1999 (PubMed).