Synaptophysin (SYP) antibody

Antigen: Synaptophysin (SYP)

Synonyms: Syn, p38, AI848995, A230093K24Rik, Syp1, syp-A, xsypi, MGC86277, syp-B, xsypii, MGC86267

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN107191

Additional Information

Alternative name: Synaptophysin

Immunogen: Synthetic human synaptophysin coupled to keyhole limpet hemocyanin.

Format: IgG-fraction, Affinity-purified

Isotype: IgG1  (Matching secondary antibodies)

Specificity: This antibody reacts with a hydrophilic peptide of 38 kD. The antibody stains neuroendocrine cells of adrenal medulla, carotid body, skin, pituitary gland, lung, and pancreas.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Pancreas. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Buffer: Affinity purified immunoglobulin fraction of rabbit antiserum against human synaptophysin containing sodium azide as preservative.

Storage: Store the antibody 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human pancreas stained with Synaptophysin antibody

Publications

Publications: Navone, Jahn, Di Gioia et al.: "Protein p38: an integral membrane protein specific for small vesicles of neurons and neuroendocrine cells." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 1, pp. 2511-27, 1987 (PubMed).

Gould, Wiedenmann, Lee et al.: "Synaptophysin expression in neuroendocrine neoplasms as determined by immunocytochemistry." in: The American journal of pathology, Vol. 126, Issue 2, pp. 243-57, 1987 (PubMed).

Kayser, Schmid, Ebert et al.: "Expression of neuroendocrine markers (neuronspecific enolase, synaptophysin and bombesin) in carcinoma of the lung." in: Pathology, research and practice, Vol. 183, Issue 4, pp. 412-7, 1988 (PubMed).

Shen, He, Powell et al.: "Recapitulation of pancreatic neuroendocrine tumors in human multiple endocrine neoplasia type I syndrome via Pdx1-directed inactivation of Men1." in: Cancer research, Vol. 69, Issue 5, pp. 1858-66, 2009 (PubMed).