TAG72 antibody

Antigen: TAG72

Clonality: Monoclonal (B72-3)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN107225

Additional Information

Alternative name: TAG-72/CA 72-4

Immunogen: BALB/C mice were injected with membrane enriched fraction of a human breast carcinoma liver metastasis.

Format: Supernatant

Isotype: IgG1 kappa  (Matching secondary antibodies)

Clone: B72-3

Specificity: This antibody is specific to an oncofetal antigen of >1,000 kD known as tumor associated glycoprotein (TAG-72) or CA 72-4. This antibody defines the mucin carried sialylated-Tn antigen. This antibody stains majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary and non-small cell lung cancer.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic, cell surface

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Tags/Labels

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human colon carcinoma stained with TAG-72 antibody