TAG72 antibody

Antigen: TAG72

Clonality: Monoclonal (CC49)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN107227

Additional Information

Alternative name: TAG-72/CA 72-4

Immunogen: BALB/C mice were injected with purified TAG-72 protein.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: CC49

Specificity: This antibody is specific to an oncofetal antigen of >1,000 kD known as tumor associated glycoprotein (TAG-72) or CA 72-4. This antibody has been shown to be distinct from B72.3 by reciprocal competition radio-immunoassays and it has a Ka higher than that of B72.3. TAG-72 is expressed in colon, breast, pancreatic, ovarian, lung and gastric cancer.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasm, cell surface

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Tags/Labels

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human colon carcinoma stained with TAG-72 antibody

Publications

Publications: Katari, Fernsten, Schlom: "Characterization of the shed form of the human tumor-associated glycoprotein (TAG-72) from serous effusions of patients with different types of carcinomas." in: Cancer research, Vol. 50, Issue 16, pp. 4885-90, 1990 (PubMed).

Sheer, Schlom, Cooper: "Purification and composition of the human tumor-associated glycoprotein (TAG-72) defined by monoclonal antibodies CC49 and B72.3." in: Cancer research, Vol. 48, Issue 23, pp. 6811-8, 1988 (PubMed).

Colcher, Minelli, Roselli et al.: "Radioimmunolocalization of human carcinoma xenografts with B72.3 second generation monoclonal antibodies." in: Cancer research, Vol. 48, Issue 16, pp. 4597-603, 1988 (PubMed).