TCR antibody (Biotin)

Antigen: TCR

Clonality: Monoclonal (536)

Host: Syrian Hamster

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN107299

Additional Information

Isotype: IgG

Clone: 536

Description: The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an a and chain in 95% of T cells, while 5% of T cells have TCRs consisting of ? and d chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.

Specificity: Anti-Mouse V? 3 TCR antibody recognizes an epitope of the variable region (V? 3) gene product. Antibody from clone 536 immunoprecipitates the TCR heterodimer with two subunits of apparent molecular mass of 52 and 42 kDa. Antigen Distribution: The antibody 536 stained less than 1% of cells in adult thymus (AKR), splenocytes, lymph node, adult CD4-8- thymocytes, and adult CD4-8-, T splenocyte. 92% of day 14 old BALB/C fetal thymus, 86% of C57BL/6 dEC (Thy-1+) and 94% of AKR dEC (Thy-1+) stained positive using this antibody.

Application Details

Application Notes: IHC (NBF/Par.), FC. Each Investigator should determine their own optimal working dilution for specific applications.

Concentration: 0.5 mg/ml

Buffer: This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative. Storage and Stability This biotinylated antibody is stable when stored at 2-8C. Do not freeze. Reporte

Storage: This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.

Research Area: Immunology, Receptors

Restrictions: For Research Use only