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anti-Telomerase catalytic subunit (hTERT) antibody

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Image for anti-Telomerase catalytic subunit (hTERT) antibody
Antigen: Telomerase catalytic subunit (hTERT)
Clonality: Polyclonal
Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)
Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)
Host: Rabbit
9 references available 9 references available
Quantity: 100ug (0.37 mg/ml (by UV absorbance a...)
Price: 265,00 $   (Plus shipping costs $25 and VAT, Invoice in EUR)
Order number: ABIN107306
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Additional Information  » anti-Telomerase catalytic subunit (hTERT) antibody

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Antigen Telomerase catalytic subunit (hTERT)
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1104-1123 of hTERT (genome data base accession number AF018167). Peptide sequence: S-R-K-L-P-G-T-T-L-T-A-L-E-A-A-A-N-P-A-L
Reactivity Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)
Clonality Polyclonal
Format Liquid (sterile filtered)
Description T elomerase is a reverse transcriptase that adds telomeric repeats (TTAGGG) n to chromosomal ends, compensating for the telomere shortening that occurs with DNA replication. In normal human somatic cells, telomerase is repressed and telomeres progressively shorten, leading to limited lifespan and senescence. Reactivation of telomerase activity is associated with human cancer and cell immortalization. Approximately 85% of human cancers, including breast, prostate, stomach, bladder, colon, and liver cancer, have telomerase activity, whereas most normal somatic cells do not. The specificity of telomerase to human cancer has led to investigations of telomerase activity and expression as a tumor marker. For example, the presence of telomerase activity in human urine has been identified as a marker for human bladder carcinoma. Human telomerase consists of three major subunits: a catalytic protein subunit called hTERT (for human TElomerase Reverse Transcriptase), a template RNA called hTR, and telomerase-associated protein (TEP-1). TERT and hTR are minimally required to reconstitute telomerase activity in vitro. In human cells, hTR is constitutively expressed, TERT transcription is a primary mechanism for regulation of telomerase activity .
Host Rabbit
Specificity This antiserum primarily detects hTERT, but several non-specific bands appear on immunoblots (Figure 1). In immunofluorescence microscopy assays, staining with anti-TERT-16 was specific to nuclei of cells with ectopic TERT expression.

Application Details  » anti-Telomerase catalytic subunit (hTERT) antibody

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Application ELISA, Western Blotting (WB), Immunoprecipitation (IP)
» Click here to view 3 available protocols!
Application Notes This affinity purified has been tested for use in immunoblotting (figure 1), immunoprecipitation (figure 2), and immunofluorescence microscopy (figure 3). In these assays, the antibody detects ectopically-expressed hTERT and high levels of endogenous hTERT. Although it has been reported that this antibody reacts against mouse TERT (mTERT) (see Drissi, et al. 2001), the binding to mTERT is considerably weaker and less specific than the binding to hTERT (not shown). A SY5Y cell nuclear extract can be used as a positive control. The anti-hTERT antibody was used for both immunoprecipitation and immunoblotting. Anti-hTERT antibody was able to immunoprecipitate TERT protein from cells with ectopic hTERT expression (lane 4). The preimmune serum was unable to immunoprecipate TERT protein (lanes 1 and 2). Recommended Dilutions : In immunoblot assays, whole cell or nuclear extracts were loaded at a concentration of 100 ug protein per well. A working dilution of 1:500 anti-TERT antibody was used followed by a 1:3,000 dilution of horseradish peroxidase- conjugated goat anti-rabbit IgG as the secondary antibody. For immunofluorescence microscopy staining, a working dilution of 1:500 was used followed by a 1:200 dilution of rhodamine-conjugated donkey anti-rabbit IgG as a secondary antibody. Immunoprecipitation was performed using 20 ul of protein A beads and 2 ul of the anti-TERT serum per 1mg protein from cell lysate. A working dilution of 1:500 is also suggested for immunohistochemistry.
Concentration 0.37 mg/ml (by UV absorbance at 280 nm)
Buffer Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Storage Store vial at -20° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening product.
Research Area DNA/RNA, Cell Cycle, Enzymes
Restrictions For Research Use only

Publications  » anti-Telomerase catalytic subunit (hTERT) antibody

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Publications Zhao, Goswami, Pokhriyal et al.: "Cyclooxygenase-2 expression during immortalization and breast cancer progression." In: Cancer research , Vol. 68, Issue 2, pp. 467-75, 2008 (PubMed)

Lee, Sung, Cheong et al.: "TERT promotes cellular and organismal survival independently of telomerase activity." In: Oncogene , Vol. 27, Issue 26, pp. 3754-60, 2008 (PubMed)

Ahmed, Passos, Birket et al.: "Telomerase does not counteract telomere shortening but protects mitochondrial function under oxidative stress." In: Journal of cell science , Vol. 121, Issue Pt 7, pp. 1046-53, 2008 (PubMed)

Natarajan, Mohan, Konopinski et al.: "Induced telomerase activity in primary aortic endothelial cells by low-LET gamma-radiation is mediated through NF-kappaB activation." In: The British journal of radiology , Vol. 81, Issue 969, pp. 711-20, 2008 (PubMed)

Sampathi, Bhusari, Shen et al.: "Human flap endonuclease I is in complex with telomerase and is required for telomerase-mediated telomere maintenance." In: The Journal of biological chemistry , Vol. 284, Issue 6, pp. 3682-90, 2009 (PubMed)

Schmelzer, Reid: "Human telomerase activity, telomerase and telomeric template expression in hepatic stem cells and in livers from fetal and postnatal donors." In: European journal of gastroenterology & hepatology , Vol. 21, Issue 10, pp. 1191-8, 2009 (PubMed)

Struauat, Liu, Rahbar et al.: "Activation of telomerase by human cytomegalovirus." In: Journal of the National Cancer Institute , Vol. 101, Issue 7, pp. 488-97, 2009 (PubMed)

Poynter, Sachs, Bright et al.: "Genetic inhibition of telomerase results in sensitization and recovery of breast tumor cells." In: Molecular cancer therapeutics , 2009 (PubMed)

Ferrario, Collotta, Carfi et al.: "Arsenic induces telomerase expression and maintains telomere length in human cord blood cells." In: Toxicology , Vol. 260, Issue 1-3, pp. 132-41, 2009 (PubMed)