Thyroglobulin (TG) antibody

Antigen: Thyroglobulin (TG)

Synonyms: TGN, TDH3, AITD3, Tgn, cog, MGC117699, TG, cb717

Clonality: Monoclonal (TGB04)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN107502

Additional Information

Immunogen: BALB/C mice were injected with purified human thyroglobulin.

Format: Ig-fraction

Isotype: IgG1

Clone: TGB04

Specificity: This antibody is specific to 330 kD protein. Thyroglobulin is synthesized by the follicular epithelial cells of the thyroid. Thyroglobulin antibody has been useful in the positive identification of thyroid carcinomas of the papillary and follicular types. This antibody cross reacts with mouse, rat and dog.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin fixed paraffin embedded tissues. No special pretreatment is required. Positive Control: Thyroid. Cellular localization: Cytoplasmic

Concentration: 100ug/ml approximately

Purification: Ig-fraction

Buffer: This antibody is supplied as purified immunoglobulin containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Hormones

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human thyroid stained with Thyroglobulin antibody

Publications

Skalli, Schuerch, Seemayer et al.: "Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 60, Issue 2, pp. 275-85, 1989 (PubMed).

Skalli, Pelte, Peclet et al.: "Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 37, Issue 3, pp. 315-21, 1989 (PubMed).

Skalli, Ropraz, Trzeciak et al.: "A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 2, pp. 2787-96, 1987 (PubMed).