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Topoisomerase (DNA) II alpha 170kDa (TOP2A) antibody

Antigen

Topoisomerase (DNA) II alpha 170kDa (TOP2A)
Synonyms TOP2, TP2A, TOP2A
Clonality Monoclonal (SWT3D1)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human
Application
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1 reference available
Catalog no. ABIN107653
Quantity
Price 454.30 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Topoisomerase II-alpha
Immunogen Synthetic polypeptide consisting of the amino acid sequence to amino acids 854-1447 of human topoisomerase II.
Format Kappa Supern.
Isotype IgG1
Clone SWT3D1
Specificity This antibody is specific to alpha form of DNA topoisomerase II. It stains the nuclei of immunoreactive cells. This antibody is useful in the staining of small cell lung cancer, non-small cell lung carcinoma and breast carcinoma.

Application Details
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Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:15-1:35 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature antigen unmasking is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Positive Control: Tonsil. Cellular localization: Nuclear
Concentration 100ug/ml approximately
Purification Kappa Supern.
Buffer This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area DNA/RNA, Cell Cycle
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications
Application Details Alternatives Variants back to top

Bauman, Holden, Brown et al.: "Differential immunohistochemical staining for DNA topoisomerase II alpha and beta in human tissues and for DNA topoisomerase II beta in non-Hodgkin's lymphomas." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 10, Issue 3, pp. 168-75, 1997 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Topoisomerase (DNA) II alpha 170kDa (TOP2A)", type "Antibodies"
Hosts Rabbit (50), Mouse (15)
Reactivities Human (64), Mouse (Murine) (9), Rat (Rattus) (8), Dog (Canine) (1), Pig (Porcine) (1)
Applications Western Blotting (WB) (55), ELISA (36), Immunohistochemistry (IHC) (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (16), Immunofluorescence (IF) (15), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (4), Flow Cytometry (FACS) (2)
Epitopes C-Term (10), Internal Region (4), AA 854-1447 (3), pSer1106,Internal Region (3), pSer1525,C-Term (3), pThr1343,C-Term (3), AA 1466-1480 (1), AA 1513-1530 (1), N-Term (1), pSer1106 (1), pSer1525 (1), pThr1343 (1)