PRICKLE4
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
FITC
Application Notes
PRICKLE4 antibody can be used for detection of PRICKLE4 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. Optimal dilutions for each application to be determined by the researcher.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
PBS containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage
4 °C/-20 °C
Storage Comment
PRICKLE4 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year.
PRICKLE4 is a member of the LIM family of proteins. It possesses two LIM domains as well as a PET domain and is thought to localize to the nucleus. Its expression in multiple malignant tissue differentiations indicate that it may play a role in cancer differentiation. Two other members of the LIM family, PRICKLE1 and PRICKLE2, are thought to function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity, suggesting the PRICKLE4 may play a similar role.