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anti-Transglutaminase II antibody

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Image for anti-Transglutaminase II antibody
Antigen: Transglutaminase II
Clonality: Monoclonal (CUB 7402)
Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Reactivity: Human, Dog (Canine), Rabbit, Guinea Pig
Host: Mouse
1 reference available 1 reference available
Quantity: 1ml (100ug/ml approximately) (Product variants)
Price: 597,65 $   (Plus shipping costs and VAT, Invoice in EUR)
Order number: ABIN107743
Availability: Ships within 5 Business days
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Additional Information  » anti-Transglutaminase II antibody

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Antigen Transglutaminase II
Immunogen BALB/C mice were injected with purified guinea pig liver TGase II.
Reactivity Human, Dog (Canine), Rabbit, Guinea Pig
Clonality Monoclonal
Format Ig-fraction
Isotype IgG1 »Matching secondary antibodies
Clone CUB 7402
Host Mouse
Specificity This antibody reacts with a 77-85 kD protein known as Transglutaminase II (TGase II). TGase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. Different tissue and cell types express varying amounts of tissue Transglutaminase. This antibody cross-reacts with dog, rabbit and Guinea pig.

Application Details  » anti-Transglutaminase II antibody

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Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
» Click here to view 1 available protocol!
Application Notes Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic, cell membrane, extracellular matrix
Concentration 100ug/ml approximately
Buffer This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.
Storage Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
Research Area Enzymes, Extracellular Matrix
Restrictions For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications  » anti-Transglutaminase II antibody

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Publications Birckbichler, Upchurch, Patterson et al.: "A monoclonal antibody to cellular transglutaminase." In: Hybridoma , Vol. 4, Issue 2, pp. 179-86, 1985 (PubMed)