Transglutaminase II antibody

Antigen: Transglutaminase II

Clonality: Monoclonal (CUB 7402)

Host: Mouse

Reactivity: Human, Dog (Canine), Rabbit, Guinea Pig

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN107743

Additional Information

Immunogen: BALB/C mice were injected with purified guinea pig liver TGase II.

Format: Ig-fraction

Isotype: IgG1  (Matching secondary antibodies)

Clone: CUB 7402

Specificity: This antibody reacts with a 77-85 kD protein known as Transglutaminase II (TGase II). TGase II catalyzes calcium-dependent post-translational modification of proteins by formation of an isopeptide bond within or between polypeptide chains. Different tissue and cell types express varying amounts of tissue Transglutaminase. This antibody cross-reacts with dog, rabbit and Guinea pig.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Cytoplasmic, cell membrane, extracellular matrix

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Enzymes, Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human breast carcinoma stained with Transglutaminase II antibody

Publications

Publications: Birckbichler, Upchurch, Patterson et al.: "A monoclonal antibody to cellular transglutaminase." in: Hybridoma, Vol. 4, Issue 2, pp. 179-86, 1985 (PubMed).