PF4 antibody (AA 31-101)

Catalog No. ABIN1077679

Product Details anti-PF4 Antibody

Target: PF4 (Platelet Factor 4 (PF4))

Binding Specificity: AA 31-101

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Platelet Factor 4 (PF4)

Specificity: The antibody is a rabbit polyclonal antibody raised against PF4. It has been selected for its ability to recognize PF4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Platelet Factor 4 (PF4) corresdonding to Ala31~Ser101 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:210-840 Immunohistochemistry: 5-20 μg/mL

1:21-84 Immunocytochemistry: 5-20 μg/mL

1:21-84 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-PF4 antibody (ABIN1077679)

Kim, Lee, Lee, Cha, Hwang: "Newly Identified HNP-F from Human Neutrophil Peptide-1 Promotes Hemostasis." in: Biotechnology journal, Vol. 14, Issue 7, pp. e1800606, (2019) (PubMed).

Target Details for PF4

Target: PF4 (Platelet Factor 4 (PF4))

Alternative Name: Platelet Factor 4 (PF4 Products)

Synonyms: CXCL4 antibody, PF-4 antibody, SCYB4 antibody, Cxcl4 antibody, Scyb4 antibody, Pf4a antibody, RATPF4A antibody, PF4A antibody, CXCL4L1 antibody, CXCL4V1 antibody, PF4-ALT antibody, SCYB4V1 antibody, PF4 antibody, platelet factor 4 antibody, platelet factor 4 variant 1 antibody, PF4 antibody, Pf4 antibody, LOC703451 antibody, PF4V1 antibody, LOC100524373 antibody, LOC102181854 antibody, LOC100630489 antibody

Background: CXCL4, SCYB4, Chemokine C-X-C-Motif Ligand 4, Oncostatin-A, Iroplact

Molecular Weight: 13.5 kDa