eIF4EBP1 antibody (AA 2-118)

Catalog No. ABIN1078005

Product Details anti-eIF4EBP1 Antibody

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Binding Specificity: AA 2-118

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1)

Specificity: The antibody is a rabbit polyclonal antibody raised against EIF4EBP1. It has been selected for its ability to recognize EIF4EBP1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1) corresdonding to Ser2-Ile118 (Accession # Q13541) with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:200-800 Immunohistochemistry: 5-20 μg/mL

1:20-80 Immunocytochemistry: 5-20 μg/mL

1:20-80 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1 Products)

Synonyms: 4E-BP1 antibody, 4EBP1 antibody, BP-1 antibody, PHAS-I antibody, 4e-bp1 antibody, AA959816 antibody, 153432_at antibody, 43-BP antibody, 4E-BP antibody, 4EBP antibody, 4e-bp antibody, 4eBP antibody, 4ebp antibody, BcDNA.HL08053 antibody, BcDNA:HL08053 antibody, CG8846 antibody, CT8705 antibody, Dm 4E-BP antibody, Dmel\\CG8846 antibody, Phas1 antibody, Thor/EIF4EBP antibody, anon-WO0172774.158 antibody, d4E-BP antibody, d4E-BP1 antibody, d4EBP antibody, d4e-bp antibody, d4eBP antibody, dthor antibody, eIF4E-BP antibody, l(2)06270 antibody, l(2)k07736 antibody, l(2)k13517 antibody, pp20 antibody, thor antibody, EIF4EBP1 antibody, fc04h09 antibody, wu:fc04h09 antibody, zgc:64137 antibody, eukaryotic translation initiation factor 4E binding protein 1 antibody, CG8846 gene product from transcript CG8846-RA antibody, EIF4EBP1 antibody, Eif4ebp1 antibody, Thor antibody, eif4ebp1 antibody

Background: 4EBP1, BP-1, PHAS-I, Phosphorylated Heat And Acid-Stable Protein Regulated By Insulin 1

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling