tyrosinase (Oculocutaneous Albinism IA) (TYR) antibody

Antigen: tyrosinase (Oculocutaneous Albinism IA) (TYR)

Synonyms: OCA1A, OCAIA, SHEP3, c, skc35, albino, sandy, zgc:109705, Tyr, C, TYR, tyr, TYRO, LOC100136546

Clonality: Monoclonal (T311)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN107987

Additional Information

Alternative name: Tyrosinase

Immunogen: BALB/C mice were injected with a recombinant tyrosinase protein.

Format: Supernatant

Isotype: IgG2a  (Matching secondary antibodies)

Clone: T311

Specificity: This antibody is specific to a cluster of proteins between 70-80 kD known as tyrosinase. This antibody does not show any cross-reaction with MAGE-1 and tyrosinase-related protein-1, TRP-1/gp75. Staining of melanomas with this antibody showed tyrosinase in melanotic as well as amelanotic variants.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. High temperature treatment of formalin-fixed tissue sections using 1mM EDTA, pH 8.0 must be performed prior to the immunostaining. Positive Control: Melanoma. Cellular localization: Cytoplasmic Other Applications: Western Blotting (1:50 to 1:100)

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union