Ubiqutin antibody

Antigen: Ubiqutin

Clonality: Monoclonal (FPM1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN108023

Additional Information

Immunogen: BALB/C mice were immunized with purified human ubiquitin.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: FPM1

Specificity: This antibody recognizes neurofibrillary tangles in Alzheimer

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Suitable for formalin/paraffin tissues. Positive Control: Alzheimer brain. Cellular localization: Nuclear, cytoplasmic and cell membrane

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing azide as the preservative.

Storage: Store the antibody 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Proteases, Amino Acids

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Lennox, Lowe, Morrell et al.: "Anti-ubiquitin immunocytochemistry is more sensitive than conventional techniques in the detection of diffuse Lewy body disease." in: Journal of neurology, neurosurgery, and psychiatry, Vol. 52, Issue 1, pp. 67-71, 1989 (PubMed).

Lowe, Lennox, Jefferson et al.: "A filamentous inclusion body within anterior horn neurones in motor neurone disease defined by immunocytochemical localisation of ubiquitin." in: Neuroscience letters, Vol. 94, Issue 1-2, pp. 203-10, 1989 (PubMed).