Red Fluorescent Protein (RFP) antibody

Details for Product No. ABIN1082200
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Synonyms RFP, RNF76
(28), (9), (4), (2), (1), (1)
(20), (19), (5), (1)
Clonality (Clone)
Monoclonal ()
(4), (2), (2), (1)
Western Blotting (WB), Immunofluorescence (IF)
(41), (33), (12), (7), (5), (5), (4), (4), (1)
Pubmed 12 references available
Quantity 100 μL
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Catalog No. ABIN1082200
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Purpose For biochemical analysis of RFP-tagged fusion proteins.
Clone 3F5
Isotype IgG2a
Specificity 3F5 efficiently recognizes monomeric red fluorescent proteins from Discosoma sp. derived from DsRed such as mRFP, mCherry or mPlum but no green fluorescent proteins.
Tested on mRFP, mCherry, mPlum, mOrange, tdTomato, DsRed
Sensitivity Sensitivity of mouse RFP antibody 3F5 goes down to 10 ng of purified RFP protein.
Characteristics Use this mouse monoclonal antibody (MAb) against monomeric RFPs, designated as RFP antibody [3F5]. The 3F5 MAb is characterized by high affinity and specificity against the DsRed derivatives and corresponding fusion proteins. It is suitable for immunoblotting assays.
Purification Hybridoma supernatant. The Hybridoma cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
Alternative Name RFP
Background Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells. In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc.) which became attractive tools for a number of applications.
Research Area Tags/Labels
Application Notes Western blot: recommended starting concentration 1/1000
IF: recommended starting concentration 1/1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
anti-Red Fluorescent Protein (RFP) antibody Western Blot: Mouse RFP antibody 3F5 is highly specific to RFPs and shows no crossreactivity to GFP and any other cellular protein. Primary antibody: 1/1000 RFP antibody 3F5, incubation for 1 h at RT. Secondary antibody: 1/500 anti-mouse-Alexa647, incubation for 1h at RT.
anti-Red Fluorescent Protein (RFP) antibody (2) Sensitivity range of mouse RFP antibody 3F5 goes down to 10 ng of purified RFP protein. 3F5 detects a band of approximately 27 kDa (predicted molecular weight of RFP: 27 kDa). Primary antibody: 1/2000 red atibody 3F5, incubation for 1 h at RT with 3% milk in TPBS. Secondary antibody: 1/500 anti-mouse-Alexa647, incubation for 1 h at RT with 3% milk in TPBS.
Product cited in: Parkinson, Baines, Keller et al.: "Calcium-dependent regulation of Rab activation and vesicle fusion by an intracellular P2X ion channel." in: Nature cell biology, Vol. 16, Issue 1, pp. 87-98, 2013 (PubMed).

Sánchez-Higueras, Sotillos, Castelli-Gair Hombría: "Common origin of insect trachea and endocrine organs from a segmentally repeated precursor." in: Current biology : CB, Vol. 24, Issue 1, pp. 76-81, 2014 (PubMed).

Whitfield, Chisholm, Hawley et al.: "A meiosis-specific form of the APC/C promotes the oocyte-to-embryo transition by decreasing levels of the Polo kinase inhibitor matrimony." in: PLoS biology, Vol. 11, Issue 9, pp. e1001648, 2013 (PubMed). (1:500).

Robles Luna, Peña, Borniego et al.: "Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein." in: Virology, Vol. 441, Issue 2, pp. 152-61, 2013 (PubMed).

Peña, Robles Luna, Zanek et al.: "Citrus psorosis and Mirafiori lettuce big-vein ophiovirus coat proteins localize to the cytoplasm and self interact in vivo." in: Virus research, Vol. 170, Issue 1-2, pp. 34-43, 2012 (PubMed).

Reichardt, Slane, El Kasmi et al.: "Mechanisms of functional specificity among plasma-membrane syntaxins in Arabidopsis." in: Traffic (Copenhagen, Denmark), Vol. 12, Issue 9, pp. 1269-80, 2011 (PubMed).

Tønnesen, Parish, Sørensen et al.: "Functional integration of grafted neural stem cell-derived dopaminergic neurons monitored by optogenetics in an in vitro Parkinson model." in: PLoS ONE, Vol. 6, Issue 3, pp. e17560, 2011 (PubMed).

Heinrich, Gascón, Masserdotti et al.: "Generation of subtype-specific neurons from postnatal astroglia of the mouse cerebral cortex." in: Nature protocols, Vol. 6, Issue 2, pp. 214-28, 2011 (PubMed).

Korzeniowski, Manjarrés, Varnai et al.: "Activation of STIM1-Orai1 involves an intramolecular switching mechanism." in: Science signaling, Vol. 3, Issue 148, pp. ra82, 2010 (PubMed).

Laxman, Sutter, Tu: "Behavior of a metabolic cycling population at the single cell level as visualized by fluorescent gene expression reporters." in: PLoS ONE, Vol. 5, Issue 9, pp. e12595, 2010 (PubMed).

Bakondi, Spees: "Human CD133-derived bone marrow stromal cells establish ectopic hematopoietic microenvironments in immunodeficient mice." in: Biochemical and biophysical research communications, Vol. 400, Issue 2, pp. 212-8, 2010 (PubMed).

Rottach, Kremmer, Nowak et al.: "Generation and characterization of a rat monoclonal antibody specific for multiple red fluorescent proteins." in: Hybridoma (2005), Vol. 27, Issue 5, pp. 337-43, 2008 (PubMed).

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