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Antibody
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anti-Vimentin VIM
Vimentin (VIM) antibody
| Antigen |
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| Synonyms |
FLJ36605, MGC102095, cb28, MGC139781, VIM, vim, Vim, vime |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Human (136), Rat (Rattus) (64), Mouse (Murine) (57), Chicken (33), Cow (Bovine) (24), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (7), Goat (5), Hamster (5), Amphibian (4), Potoroo (2), All Species (1), Fish (1), Primate (1), Rabbit (1), Xenopus laevis (1)
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Application
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Western Blotting (WB) (92), Immunofluorescence (IF) (62), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (57), Flow Cytometry (FACS) (48), Immunohistochemistry (IHC) (40), ELISA (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Enzyme Immunoassay (EIA) (15), Immunoprecipitation (IP) (13), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (EM) (2), Dot Blot (Dot) (1)
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| Catalog no. |
ABIN108227 |
| Quantity |
1ml (100ug/ml approximately) (Variants) |
| Price |
298.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business days |
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Alternative name
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Vimentin
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Immunogen
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Recombinant human vimentin protein.
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Format
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Ig-fraction, Purified
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Specificity
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This antibody reacts with a 57- 60 kD protein. Vimentin is the main intermediate filament protein in mesenchymal cells and therefore is of value in the differential diagnosis of undifferentiated neoplasms.
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Application Notes
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Titer/Working Dilution: This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. No special treatment is required for formalin fixed paraffin embedded tissues. Positive Control: Sarcomas, melanomas. Cellular localization: Cytoplasmic
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Concentration
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100ug/ml approximately
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Buffer
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Purified immunoglobulin fraction of rabbit antiserum against Vimentin containing sodium azide as preservative.
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Storage
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Research Area
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Cell/Tissue Markers, Cytoskeleton
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Restrictions
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For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union
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Alternatives for antigen "Vimentin (VIM)", type "Antibodies"
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Hosts
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Rabbit (81), Mouse (66), Chicken (9), Guinea Pig (2), Goat (1)
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Reactivities
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Human (136), Rat (Rattus) (64), Mouse (Murine) (57), Chicken (33), Cow (Bovine) (24), Pig (Porcine) (23), Dog (Canine) (9), Mammalian (8), Monkey (7), Goat (5), Hamster (5), Amphibian (4), Potoroo (2), All Species (1), Fish (1), Primate (1), Rabbit (1), Xenopus laevis (1)
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Applications
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Western Blotting (WB) (92), Immunofluorescence (IF) (62), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (57), Flow Cytometry (FACS) (48), Immunohistochemistry (IHC) (40), ELISA (31), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Enzyme Immunoassay (EIA) (15), Immunoprecipitation (IP) (13), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (EM) (2), Dot Blot (Dot) (1)
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Conjugates
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FITC (8), Biotin (6), PE (4), Alexa Flour 488 (2), Alexa Fluor 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2), DY547 (1), Dyomics 547 (1)
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Epitopes
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pSer82 (15), C-Term (2), Center (2), S82 (2), Ab-56 (1), Ab-83 (1), pSer56 (1), pSer83 (1)
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Alternatives 
