X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3) antibody

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3)

Synonyms: AI182522, AW537713, 4432412E01Rik, MGC69118, im:7142103, zgc:101608, si:dkey-11b8.1, xrcc3, XRCC3, MGC142843

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN108329

Additional Information

Alternative name: X-Ray Repair Cross Complementing (XRCC3)

Immunogen: A synthetic peptide derived from the N-terminus of human XRCC3 protein.

Format: Ig-fraction, Purified

Specificity: This antibody reacts with a 32 kDa protein, known as XRCC3 (X-Ray Cross Complementing 3). XRCC3 is essential for repairing the double strand DNA breaks by homologous recombination between sister chromatids. It is homologous to Rad1. Five human homologues of RAD 51, XRCC2, XRCC3, RAD51B, RAD51C, RAD51D, interact among themselves to form one big complex or several smaller complexes.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Testis. Cellular localization: Nuclear, cytoplasmic Other Applications: Western Blotting (1:100-500).

Concentration: 100ug/ml approximately

Purification: Ig-fraction,Purified

Buffer: Purified immunoglobulin fraction of rabbit antiserum against human XRCC3 containing sodium azide as preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: DNA/RNA

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Tambini, George, Rommens et al.: "The XRCC2 DNA repair gene: identification of a positional candidate." in: Genomics, Vol. 41, Issue 1, pp. 84-92, 1997 (PubMed).

Johnson, Liu, Jasin: "Mammalian XRCC2 promotes the repair of DNA double-strand breaks by homologous recombination." in: Nature, Vol. 401, Issue 6751, pp. 397-9, 1999 (PubMed).