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C3 antibody (Complement Component 3) (C-Term)

Details for Product anti-C3 Antibody No. ABIN108480, Supplier: Log in to see
Antigen
  • AHUS5
  • AI255234
  • ARMD9
  • asp
  • ASP
  • c3
  • C3
  • C3-1
  • C3a
  • C3b
  • C3d
  • c3_D
  • CPAMD1
  • cpamd1
  • HSE-MSF
  • Plp
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
Alternatives
anti-Human C3 antibody for ELISA
Epitope
C-Term
13
11
6
5
5
4
3
3
3
3
2
2
1
1
1
1
Reactivity
Human
245
67
56
18
13
5
5
5
3
2
1
Host
Mouse
142
103
86
25
13
8
Clonality (Clone)
Monoclonal ()
Conjugate
This C3 antibody is un-conjugated
34
31
20
6
6
5
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
1
Application
ELISA, Western Blotting (WB)
194
191
72
70
61
47
46
43
42
35
33
26
22
5
4
1
1
1
1
1
1
1
Supplier
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Immunogen
  • The antibody is a mouse monoclonal antibody against human peptide.
  • Source of Immunogen: Peptide
Sequence The human peptide with amino acid sequence RASHLGLA (which is located in C-terminal part of the human ASP).
Clone 4H3
Isotype IgG1
No Cross-Reactivity Human
Cross-Reactivity (Details) Not yet tested in other species.
Purification Affinity chromatography on a column with immobilized protein G.
Alternative Name Acylation Stimulating Protein (ASP) (C3 Antibody Abstract)
Background
  • Acylation Stimulating Protein (ASP, also known as C3a desArg) is one of activation fragments formed from the activation of complement cascade. ASP is produced through a process involving three proteins: C3, factor B and adipsin, which are secreted by adipocytes. Interactions of C3 with factor B and with adipsin result in production of C3a followed by desargination of the carboxyl terminus to generate ASP (C3a desArg). Human ASP contains 77 amino acids with 6 cysteins involved in disulfide bridges between residues 22-49, 23-56 and 36-57. ASP is a highly cationic molecule containing no carbohydrate. ASP has a primary role in regulation of lipid metabolism in adipocytes, where it stimulates glucose uptake, increase the activity of diacylglycerol acyltransferase, and inhibits hormone-sensitive lipase activity. In cellular studies, ASP increases fat storage through increased triglyceride synthesis and decreased intracellular lipolysis. In animal models, ASP deficient mice demonstrate reduced body weight, reduced leptin and reduced adipose tissue mass and ASP deficiency results in protection against development of obesity. In human, a number of studies have shown the relationship between ASP, obesity, diabetes and dyslipidemia. It was reported that concentration of circulating ASP is positively related to body adiposity and decreases after weight loss. Because ASP enhances triglyceride storage, whereas interfering with ASP production reduces body fat and protects against diet-induced obesity and insulin resistance, reducing the production of ASP and ASP receptor antagonists represents potential approaches for treating obesity and type 2 diabetes.
  • Other Names: ASP
  • Research Areas: Energy metabolism and body weight regulation
Pathways Complement System
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Background publications Cianflone K,Phelis S,Davignon J,Gilfix BM: "ApoE phenotype influences plasma ASP in hyperapoB subjects." in: Atherosclerosis, Vol. 170, Issue 2, pp. 285-91, 2003 (PubMed).

Faraj M,Havel PJ,Phelis S,Blank D,Sniderman AD,Cianflone K: "Plasma acylation-stimulating protein, adiponectin, leptin, and ghrelin before and after weight loss induced by gastric bypass surgery in morbidly obese subjects." in: J Clin Endocrinol Metab, Vol. 88, Issue 4, pp. 1594-602, 2003 (PubMed).

Matthan NR,Cianflone K,Lichtenstein AH,Ausman LM,Jauhiainen M,Jones PJ: "Hydrogenated fat consumption affects acylation-stimulating protein levels and cholesterol esterification rates in moderately hypercholesterolemic women." in: J Lipid Res, Vol. 42, Issue 11, pp. 1841-8, 2001 (PubMed).