anti-Clusterin antibody (CLU)

Details for Product anti-CLU Antibody No. ABIN108488
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Antigen
Synonyms
LOC100136120, clu, MGC53055, zgc:73352, xclu, MGC89163, CLU, APO-J, APOJ, CLI, CLU1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, DAG, RATTRPM2B, SP40, TRPM2B, Trpm2, Trpmb, AI893575, ApoJ, ... show more
LOC100136120, clu, MGC53055, zgc:73352, xclu, MGC89163, CLU, APO-J, APOJ, CLI, CLU1, CLU2, KUB1, NA1/NA2, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, DAG, RATTRPM2B, SP40, TRPM2B, Trpm2, Trpmb, AI893575, ApoJ, Cli, D14Ucla3, Sgp-2, Sgp2, Sugp-2, GP80, gpIII, 40 show less
Reactivity
Human
(259), (62), (56), (24), (19), (8), (4), (2), (2)
Host
Mouse
(167), (109), (74), (9), (5), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
This Clusterin antibody is un-conjugated
(35), (29), (14), (10), (9), (9)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(296), (248), (127), (52), (36), (33), (29), (27), (18), (16), (11), (9), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Immunogen
  • The antibody is a mouse monoclonal antibody against Human Clusterin.
  • Source of Immunogen: The antibody was prepared against Triton X-100 human sperm extract.
Clone Hs-3
Isotype IgG1
No Cross-Reactivity Cat (Feline), Dog (Canine), Cow (Bovine)
Cross-Reactivity (Details) Not yet tested in other species.
Purification Affinity chromatography on a column with immobilized protein A-Sepharose followed by DEAE-chromatography.
Alternative Name Clusterin (CLU Antibody Abstract)
Background
  • Clusterin is a 75-80 kD disulfide-linked heterodimeric protein containing about 30 % of N-linked carbohydrate rich in sialic acid, but truncated forms targeted to the nucleus have also been identified. The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the alpha and beta chains. These are assembled anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil alpha-helices and three predicted amphipathic alpha-helices. The six sites of N-linked glycosylation are indicated as yellow spots. Across a broad range of species clusterin shows 70 % to 80 % of sequence homology. It is ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodelling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. A detailed mechanism of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clus-terin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
Research Area Receptors, Cancer
Application Notes
  • Quality control: SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2, 15 mM SODIUM AZIDE.
Preservative Sodium azide
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
General Zenkel, Kruse, Jünemann et al.: "Clusterin deficiency in eyes with pseudoexfoliation syndrome may be implicated in the aggregation and deposition of pseudoexfoliative material." in: Investigative ophthalmology & visual science, Vol. 47, Issue 5, pp. 1982-90, 2006 (PubMed).

Zhou W, Janulis L, Park II et al.: "A novel anti-proliferative property of clusterin in prostate cancer cells." in: Life Sci, Vol. 72, Issue 1, pp. 11-21, 2002 (PubMed).

Jones, Jomary: "Clusterin." in: The international journal of biochemistry & cell biology, Vol. 34, Issue 5, pp. 427-31, 2002 (PubMed).

Connor JR, Kumar S, Sathe G et al.: "Clusterin expression in adult human normal and osteoarthritic articular cartilage." in: Osteoarthritis Cartilage, Vol. 9, Issue 8, pp. 727-37, 2001 (PubMed).

Validation Images
Catalog No. ABIN108488
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