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Leptin antibody (LEP)

Details for Product anti-LEP Antibody No. ABIN108489, Supplier: Log in to see
Antigen
  • ob
  • obese
  • LEPD
  • OB
  • OBS
Alternatives
anti-Human Leptin antibody for Western Blotting
Reactivity
Human
331
151
131
18
3
1
1
Host
Mouse
349
133
20
8
4
Clonality (Clone)
Monoclonal ()
Conjugate
This Leptin antibody is un-conjugated
60
19
13
7
7
5
5
3
3
3
3
3
3
3
3
2
2
Application
Western Blotting (WB)
337
321
69
39
39
33
26
25
17
17
16
13
10
7
5
3
3
3
2
2
1
1
1
1
1
1
Supplier
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Immunogen
  • The antibody is a mouse monoclonal antibody against human recombinant Human Leptin.
  • Source of Immunogen: E. coli
Clone LEP-02
Isotype IgG1 kappa
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Not yet tested in other species.
Purification Affinity chromatography on a column with immobilized protein G.
Alternative Name Leptin (LEP Antibody Abstract)
Background
  • Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.
  • Other Names: Obesity factor, Obese protein, LEP, OB, OBS
  • Research Areas: Animal studies, Energy metabolism and body weight regulation, Reproduction
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Background publications Considine, Sinha, Heiman et al.: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996 (PubMed).

Zhang, Proenca, Maffei et al.: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994 (PubMed).

Halaas, Gajiwala, Maffei et al.: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995 (PubMed).

Lönnqvist, Arner, Nordfors et al.: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995 (PubMed).

Maffei, Halaas, Ravussin et al.: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995 (PubMed).