anti-Leptin Receptor antibody (LEPR)

Details for Product anti-LEPR Antibody No. ABIN108490
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Antigen
Synonyms obr, cd295, ob-rb, CD295, LEP-R, LEPRD, OB-R, OBR, ObRb, LEPROT, Leprb, Modb1, OB-RGRP, Obr, db, diabetes, obese-like, obl, Ob-R, Ob-RM, Fa, LEPR, Obrgrp, VPS55
Reactivity
Human
(170), (60), (50), (12), (8), (2), (1), (1), (1), (1), (1)
Host
Mouse
(130), (30), (28), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
This Leptin Receptor antibody is un-conjugated
(9), (9), (8), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3)
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
(130), (103), (43), (31), (29), (23), (7), (7), (6), (3), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Immunogen
  • The antibody is a mouse monoclonal antibody against recombinant Human Leptin Receptor. The Human Leptin Receptor is a recombinant protein produced in mouse myeloma cell line. DNA sequence including the extracellular domain of Leptin Receptor (amino acid residues 1 839) fused to the Fc region of human IgG (with IIEGR added at the amino terminus and 6 histidine residues added at the carboxy terminus).
  • Source of Immunogen: NSO mouse myeloma cell line
Clone LPR-01
Isotype IgG1 kappa
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Not yet tested in other species.
Purification Affinity chromatography on a column with immobilized protein G.
Alternative Name Leptin Receptor (LEPR Antibody Abstract)
Background
  • Leptin receptor (OB R) was identified as a leptin binding protein (Leptin, the product of the ob gene, is a single chain 16 kDa protein consisting of 146 amino acid residues.) OB R was found to be a member of the class I cytokine receptor family with a large extracellular domain comprising 816 amino acid residues. Leptin receptor exists in multiple forms with a common extracellular domain and a variable length cytoplasmatic portion. Alternate splicing from a single gene derives the six isoforms of the Leptin receptor. The soluble form of the Leptin receptor, OB R contains no intracellular motifs or transmembrane residues, thus it consists entirely of the extracellular ligand binding domain of the receptor. Long forms of OB-R transcripts were reported to be expressed predominantly in regions of the hypothalamus which provides evidence that Leptin receptor is important in body weight regulation. Expression of short forms of OB-R transcripts have been found in multiple tissues, including the choroid plexus, lung, kidney, and primitive hematopoietic cell populations. Leptin receptor may act as a negative regulator of Leptin activity and it may maintain a pool of available bioactive Leptin by binding and delaying its clearance from circulation. Soluble Leptin receptor levels are indirectly proportional to adiposity and are increased in females versus males. Leptin receptor levels are highest in infants, decrease into adolescence, and remain relatively stable throughout adulthood. Soluble Leptin receptor is also found upregulated in patients with chronic heart failure, end-stage renal disease and anorexia.
  • Research Areas: Animal studies, Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Reproduction
Research Area Cardiovascular, Fatty Acids, Hormones, Receptors
Application Notes
  • Quality control: SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
General Wang Y, Kuropatwinski KK, White DW et al.: "Leptin receptor action in hepatic cells." in: J Biol Chem, Vol. 272, Issue 26, pp. 16216-23, 1997 (PubMed).

Campfield LA, Smith FJ, Burn P: "The OB protein (leptin) pathway--a link between adipose tissue mass and central neural networks." in: Horm Metab Res, Vol. 28, Issue 12, pp. 619-32, 1996 (PubMed).

Cioffi, Shafer, Zupancic et al.: "Novel B219/OB receptor isoforms: possible role of leptin in hematopoiesis and reproduction." in: Nature medicine, Vol. 2, Issue 5, pp. 585-9, 1996 (PubMed).

Tartaglia, Dembski, Weng et al.: "Identification and expression cloning of a leptin receptor, OB-R." in: Cell, Vol. 83, Issue 7, pp. 1263-71, 1996 (PubMed).

Validation Images
Catalog No. ABIN108490
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