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EPCAM antibody (Epithelial Cell Adhesion Molecule)

Details for Product anti-EPCAM Antibody No. ABIN108496, Supplier: Log in to see
Antigen
  • sb:cb6
  • tacstd
  • wu:fj17g02
  • zgc:110304
  • zgc:77119
  • tacstd1
  • MGC80540
  • EPCAM
  • TACSTD1
  • egp
  • ksa
  • m4s1
  • mk-1
  • cd326
  • egp40
  • mic18
  • trop1
  • ep-cam
  • hegp-2
  • co17-1a
  • ga733-2
  • GP40
  • LEU-9
  • TP41
  • Tp40
  • DIAR5
  • EGP-2
  • EGP314
  • EGP40
  • ESA
  • HNPCC8
  • KS1/4
  • KSA
  • M4S1
  • MIC18
  • MK-1
  • TROP1
  • CD326
  • EGP
  • Egp314
  • Ep-CAM
  • EpCAM1
  • GA733-2
  • Ly74
  • Tacsd1
  • Tacstd1
  • gp40
Reactivity
Human
439
91
45
2
1
1
1
Host
Mouse
335
127
39
1
Clonality (Clone)
Monoclonal ()
Conjugate
This EPCAM antibody is un-conjugated
34
23
16
13
6
5
5
4
4
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
288
211
131
121
117
117
68
50
39
13
8
5
5
4
4
4
2
2
2
1
1
1
1
1
Supplier
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Immunogen
  • The antibody is a mouse monoclonal antibody against recombinant Human Ep-CAM.
  • Source of Immunogen: E.coli
Clone MK-1-410
Isotype IgG1
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Not yet tested in other species.
Purification Affinity chromatography on a column with immobilized protein G.
Alternative Name MK1/Ep CAM (EPCAM Antibody Abstract)
Background
  • The Ep-CAM antigen (Epithelial Cell Adhesion Molecule), also termed as MK-1, GA733-2, KSA, 17-1A or EGP40, is a 40 kDa type-I transmembrane glycoprotein with properties of Ca2+ -independent homotypic intracellular adhesion molecule. The full leghth of the molecule is 314 amino acids. Structurally, Ep-CAM is not related to any of the major families of adhesion molecules. The Ep-CAM antigen is overexpessed on the majority of tumor cells of most human epithelia of variety of tumor tissues such as stomach, colon, pancreas, gall bladder, bile duct, mammary gland, breast and lung carcinoma. Ep-CAM has been suggested to be involved in the differentiation and growth of epithelial cells under normal physiological conditions through its homotypic cell-cell adhesion activity. As it is overexpressed on most carcinomas, Ep-CAM antigen has been used as a target for diagnosis and therapy of cancer.
Pathways
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Background publications Abe, Kuroki, Imakiire et al.: "Preparation of recombinant MK-1/Ep-CAM and establishment of an ELISA system for determining soluble MK-1/Ep-CAM levels in sera of cancer patients." in: Journal of immunological methods, Vol. 270, Issue 2, pp. 227-33, 2002 (PubMed).

Balzar, Briaire-de Bruijn, Rees-Bakker et al.: "Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions." in: Molecular and cellular biology, Vol. 21, Issue 7, pp. 2570-80, 2001 (PubMed).

Balzar, Winter, de Boer et al.: "The biology of the 17-1A antigen (Ep-CAM)." in: Journal of molecular medicine (Berlin, Germany), Vol. 77, Issue 10, pp. 699-712, 2000 (PubMed).