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Transferrin Receptor (TFR) (Soluble) antibody

Details for Product No. ABIN108500, Supplier: Log in to see
Antigen
  • 2610028K12Rik
  • AI195355
  • AI426448
  • AU015758
  • CD71
  • E430033M20Rik
  • Mtvr-1
  • Mtvr1
  • TFR
  • TFR1
  • TR
  • Trfr
  • p90
  • transferrin receptor (p90, CD71)
  • transferrin receptor
  • TFRC
  • Tfrc
Alternatives
anti-Human Transferrin Receptor antibody for ELISA
Epitope
Soluble
16
10
10
8
7
6
6
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
203
78
44
10
6
4
4
3
3
2
1
1
1
1
1
Host
Mouse
156
87
35
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
29
28
20
7
5
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
133
130
103
55
46
36
31
30
18
13
13
7
3
3
1
1
Options
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Immunogen
  • The antibody is a mouse monoclonal antibody against Human sTfR.
  • Source of Immunogen: Human placenta
Clone TFR-01
Isotype IgG1
No Cross-Reactivity Human
Cross-Reactivity (Details) Not yet tested in other species.
Purification Affinity chromatography on a column with immobilized protein G.
Alternative Name Transferrin Receptor (TFR Antibody Abstract)
Background
  • The transferrin receptor (TfR) is the gateway for transferrin-bound-iron entering all body cells. TfR is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body TfR content. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein, it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The serum sTfR concentration reflects the total body mass of cellular transferrin receptor. Anaemias associated with enhanced erythropoiesis and iron deficiency result in an elevation in the sTfR values. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in its decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.
  • Other Names: TfR
  • Research Areas: Iron metabolism, Oncology
Pathways Transition Metal Ion Homeostasis
Application Notes
  • Quality control: Western Blot - to confirm specifity of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Background publications Cotton F,Thiry P,Boeynaems J: "Measurement of soluble transferrin receptor by immunoturbidimetry and immunonephelometry." in: Clin Biochem, Vol. 33, Issue 4, pp. 263-7, 2000 (PubMed).

De Block CE,Van Campenhout CM,De Leeuw IH,Keenoy BM,Martin M,Van Hoof V,Van Gaal LF: "Soluble transferrin receptor level: a new marker of iron deficiency anemia, a common manifestation of gastric autoimmunity in type 1 diabetes." in: Diabetes Care, Vol. 23, Issue 9, pp. 1384-8, 2000 (PubMed).

Olivares M,Walter T,Cook JD,Hertrampf E,Pizarro F: "Usefulness of serum transferrin receptor and serum ferritin in diagnosis of iron deficiency in infancy." in: Am J Clin Nutr, Vol. 72, Issue 5, pp. 1191-5, 2000 (PubMed).

Hikawa A,Nomata Y,Suzuki T,Ozasa H,Yamada O: "Soluble transferrin receptor-transferrin complex in serum: measurement by latex agglutination nephelometric immunoassay." in: Clin Chim Acta, Vol. 254, Issue 2, pp. 159-72, 1996 (PubMed).

Flowers CH,Skikne BS,Covell AM,Cook JD: "The clinical measurement of serum transferrin receptor." in: J Lab Clin Med, Vol. 114, Issue 4, pp. 368-77, 1989 (PubMed).