anti-Adiponectin antibody (Adiponectin, C1Q and Collagen Domain Containing)

Details for Product anti-ADIPOQ Antibody No. ABIN108503
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Antigen
Synonyms MGC84292, ADIPOQ, APM1, ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, GBP28, 30kDa, APN, Acdc, Acrp30, adipo, apM1, ADN
Reactivity
Mouse (Murine)
(430), (138), (88), (19), (11), (4), (3), (2), (2)
Host
Rabbit
(308), (238), (12), (12), (4), (3)
Clonality
Polyclonal
Conjugate
This Adiponectin antibody is un-conjugated
(59), (23), (18), (1), (1), (1), (1)
Application
Western Blotting (WB)
(393), (384), (69), (46), (43), (35), (16), (14), (13), (11), (8), (7), (7), (4), (4), (4), (2), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Immunogen
  • The antibody was raised in rabbits by immunization with the recombinant Murine Adiponectin.
  • Source of Immunogen: HEK293
Sequence EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Not yet tested in other species.
Purification Immunoaffinity chromatography on a column with immobilized mouse recombinant Adiponectin.
Alternative Name Adiponectin (ADIPOQ Antibody Abstract)
Background
  • Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 aminoacid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells. The protein belongs to the soluble defence collagen superfamily, it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum. Together, these complexes make up approximately 0.01 % of total serum protein. Adiponectin receptors AdipoR1 and AdipoR2 have been recently cloned, AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. Paradoxically, adipose tissue-expressed adiponectin levels are inversely related to the degree of adiposity. Adiponectin concentrations correlate negatively with glucose, insulin, triglyceride concentrations, liver fat content and body mass index and positively with high-density lipoprotein-cholesterol levels, hepatic insulin sensitivity and insulin-stimulated glucose disposal. Adiponectin has been shown to increase insulin sensitivity and decrease plasma glucose by increasing tissue fat oxidation. Of particular interest is that low adiponectin serum levels predict type 2 diabetes independent of other risk factors. Adiponectin also inhibits the inflammatory processes of atherosclerosis suppressing the expression of adhesion and cytokine molecules in vascular endothelial cells and macrophages, respectively. This adipokine plays a role as a scaffold of newly formed collagen in myocardial remodelling after ischaemic injury and also stimulates angiogenesis by promoting cross-talk between AMP-activated protein kinase and Akt signalling in endothelial cells. Low serum adiponectin levels are found in patients with coronary artery disease. Moreover, high circulating levels of adiponectin are associated with decreased risk of myocardial infarction, independent of other factors. Altogether, adiponectin has the potential to become a clinically relevant parameter to be measured routinely in subjects at risk for type 2 diabetes, atherosclerosis and the metabolic syndrome.
Research Area Cancer, Atherosclerosis, Metabolism, Hormones
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
General Xu, Wang, Keshaw et al.: "The fat-derived hormone adiponectin alleviates alcoholic and nonalcoholic fatty liver diseases in mice." in: The Journal of clinical investigation, Vol. 112, Issue 1, pp. 91-100, 2003 (PubMed).

Yamauchi, Kamon, Minokoshi et al.: "Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase." in: Nature medicine, Vol. 8, Issue 11, pp. 1288-95, 2002 (PubMed).

Wang, Xu, Knight et al.: "Hydroxylation and glycosylation of the four conserved lysine residues in the collagenous domain of adiponectin. Potential role in the modulation of its insulin-sensitizing activity." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19521-9, 2002 (PubMed).

Berg, Combs, Du et al.: "The adipocyte-secreted protein Acrp30 enhances hepatic insulin action." in: Nature medicine, Vol. 7, Issue 8, pp. 947-53, 2001 (PubMed).

Validation Images
Catalog No. ABIN108503
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