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Agouti Related Protein Homolog (Mouse) (AGRP) antibody

Details for Product No. ABIN108504, Supplier: Log in to see
Antigen
  • Agrt
  • Art
  • AGRT
  • ART
  • ASIP2
Reactivity
Human
61
26
15
4
4
1
1
Host
Rabbit
66
23
6
2
1
Clonality
Polyclonal
Conjugate
Un-conjugated
12
5
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
64
52
33
7
6
4
4
2
2
2
2
1
1
1
Supplier
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Immunogen
  • The antibody was raised in rabbits by immunization with the recombinant Human Agouti-Related Protein.
  • Source of Immunogen: E. coli
Sequence The immunization antigen (14.4 kDa) is a protein containing 128 AA of recombinant Human Agouti-Related Protein. N-Terminal His-tag, 16 extra AA. MKHHHHHHHM LVPRGSAQMG LAPMEGIRRP DQALLPELPG LGLRAPLKKT TAEQAEEDLL QEAQALAEVL DLQDREPRSS RRCVRLHESC LGQQVPCCDP CATCYCRFFN AFCYCRKLGT AMNPCSRT
No Cross-Reactivity Human
Cross-Reactivity (Details) Not yet tested in other species.
Purification Immunoaffinity chromatography on a column with immobilized recombinant Human Agouti-Related Protein.
Alternative Name Agouti Related Protein (AGRP Antibody Abstract)
Background
  • Agouti-related protein is an endogenous antagonist of hypothalamic alpha-melanocortin receptors MC3R and MC4R with potent orexigenic activity. Although a complete deletion of the AGRP gene does not produce any significant metabolic phenotypes, reduction in AGRP expression by RNA interference is associated with increased metabolic rate along with reduced weight gain. In hypothalamus, it is produced by neurons in the medial portion of arcuate nucleus, which produce also the potent orexigenic peptide Neuropeptide Y (NP-Y). Another site of central AGRP production is the hypothalamic nucleus. AGRP encompasses 132 amino acid residues and its alpha-melanocortin inhibiting activity results in a 34 amino acid cystine knot domain within the C-terminal (87-132) portion of the protein. Both AGRP and NP-Y expression was shown to be supressed by leptin. Central administration of AGRP induces hyperphagia and increased gain in body weight in rodents, but may also exert metabolic effects even when hyperphagia is prevented. In the absence of hyperphagia, intracerebral-ventricular administration of AGRP caused significant increases in plasma leptin and insulin concentrations (two-fold and 1.5-fold, respectively) and fat pad mass. In the perifery, AGRP mRNA was found in adrenal glands, lung, testis, ovary, skeletal muscle and adipose tissue in humans or rodents. In the adrenals, it was shown that AGRP antagonizes glucosteroid production mediated by MC4R. AGRP could then modulate locally the functions of some peripheral tissues such as adrenals. In human and rat serum, detectable levels of AGRP-like activity were reported in the lower picogram range. The serum AGRP levels were elevated in obese humans compared to lean controls and increased with fasting in rats.
  • Other Names: AGRP, AGRT, ART
  • Research Areas: Energy metabolism and body weight regulation
Pathways
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.05 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Background publications Song Y, Golling G, Thacker TL et al.: "Agouti-related protein (AGRP) is conserved and regulated by metabolic state in the zebrafish, Danio rerio." in: Endocrine, Vol. 22, Issue 3, pp. 257-65, 2003 (PubMed).

Mizuno TM, Mobbs CV: "Hypothalamic agouti-related protein messenger ribonucleic acid is inhibited by leptin and stimulated by fasting." in: Endocrinology, Vol. 140, Issue 2, pp. 814-7, 1999 (PubMed).

Ollmann MM, Lamoreux ML, Wilson BD et al.: "Interaction of Agouti protein with the melanocortin 1 receptor in vitro and in vivo." in: Genes Dev, Vol. 12, Issue 3, pp. 316-30, 1998 (PubMed).