Collagen, Type XVIII, alpha 1 (COL18A1) antibody

Antigen: Collagen, Type XVIII, alpha 1 (COL18A1)

Synonyms: KNO, KNO1, FLJ27325, FLJ34914, MGC74745, kno, MGC76284, COL18A1, MGC140499, endostatin, cb371, cb529, fc96d06, sb:cb529, wu:fb47h10, wu:fb77g12, wu:fc50f04, wu:fc96d06, wu:fk02h12

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN108513

Additional Information

Alternative name: Endostatin

Format: Affinity-purified

Description: Specification: Anti-Human Endostatin Antibody was raised in rabbit against recombinant human Endostatin. Recombinant Human Endostatin produced in Pichia pastoris is a single, glycosylated, polypeptide having a total molecular mass of 20,000 Dalton C-terminal fragment of collagen XVIII.

Application Details

Application Notes: Titre: Is defined by indirect ELISA, it is >1/100,000 for antibody concentration 1mg/ml, 25 ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve.

Storage: Store lyophilized antibody at 2-8°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time, it does not show decline in activity after two weeks at 4°C.

Research Area: Hormones

Restrictions: For Research Use only

Publications

Publications: Rehn M, Pihlajaniemi T: "Alpha 1(XVIII), a collagen chain with frequent interruptions in the collagenous sequence, a distinct tissue distribution, and homology with type XV collagen." in: Proc Natl Acad Sci U S A, Vol. 91, Issue 10, pp. 4234-8, 1994 (PubMed).

O'Reilly MS, Boehm T, Shing Y et al.: "Endostatin: an endogenous inhibitor of angiogenesis and tumor growth." in: Cell, Vol. 88, Issue 2, pp. 277-85, 1997 (PubMed).

Sertie AL, Sossi V, Camargo AA et al.: "Collagen XVIII, containing an endogenous inhibitor of angiogenesis and tumor growth, plays a critical role in the maintenance of retinal structure and in neural tube closure (Knobloch syndrome)." in: Hum Mol Genet, Vol. 9, Issue 13, pp. 2051-8, 2000 (PubMed).

Karumanchi SA, Jha V, Ramchandran R et al.: "Cell surface glypicans are low-affinity endostatin receptors." in: Mol Cell, Vol. 7, Issue 4, pp. 811-22, 2001 (PubMed).