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Collagen, Type XVIII, alpha 1 (COL18A1) antibody

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Synonyms kno, kno1, cb371, cb529, fc96d06, sb:cb529, wu:fb47h10, wu:fb77g12, wu:fc50f04, wu:fc96d06, wu:fk02h12, KNO, KNO1, KS
(120), (48), (24)
(112), (21), (3), (1)
(8), (8), (8), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1)
ELISA, Western Blotting (WB)
(94), (91), (50), (22), (11), (7), (6), (5), (2), (1), (1)
Pubmed 4 references available
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Quantity 0.1 mg
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  • The antibody was raised in rabbits by immunization with the recombinant Human Endostatin. The immunization antigen (20 kDa) is a terminal fragment of collagen XVIII.
  • Source of Immunogen: Pichia pastoris
No Cross-Reactivity Human
Cross-Reactivity (Details) Not yet tested in other species.
Purification Immunoaffinity chromatography on a column with immobilized recombinant Human Endostatin.
Alternative Name Endostatin (COL18A1 Antibody Abstract)
  • Endostatin is a 20 kDa C-terminal fragment of collagen XVIII. The exact molecular mass determined by ESI-MS was found to be 18 494 Da.Endostatin specifically inhibits. endothelial proliferation and potently inhibits angiogenesis and tumor growth. Anti- endostatin monoclonal antibody administered to nude mice transplanted with human hepatocellular carcinoma cells (JHH-1 line) that endogenously produced endostatin promoted tumor angiogenesis by inhibiting endostatin activity in the tumor and subsequently increased tumor mass by preventing cancer cells from undergoing apoptosis. The ability of endostatin to bind Zn2+ is essential for its antiangiogenic activity. The endostatin precursor collagen XVIII is expressed at high levels in human livers, the main source being hepatocytes. The generation of endostatin or endostatin-like collagen XVIII fragments is catalyzed by proteolytic enzymes, including cathepsin L and matrix metalloproteases, that cleave peptide bonds within the protease-sensitive hinge region of the C-terminal domain. The processing of collagen XVIII to endostatin may represent a local control mechanism for the regulation of angiogenesis. Circulating endostatin was detected in patients with various types of cancer as well as in healthy controls. Circulating endostatin concentrations are significantly increased in patients with systemic sclerosis. Interestingly, pericardial fluid levels of endostatin are associated with the presence (40 % lower levels) or absence of collaterals in patients with CAD.
  • Research Areas: Oncology
Research Area Cancer, Angiogenesis, Extracellular Matrix, Hormones
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Background publications Karumanchi, Jha, Ramchandran et al.: "Cell surface glypicans are low-affinity endostatin receptors." in: Molecular cell, Vol. 7, Issue 4, pp. 811-22, 2001 (PubMed).

Sertie AL, Sossi V, Camargo AA et al.: "Collagen XVIII, containing an endogenous inhibitor of angiogenesis and tumor growth, plays a critical role in the maintenance of retinal structure and in neural tube closure (Knobloch syndrome)." in: Hum Mol Genet, Vol. 9, Issue 13, pp. 2051-8, 2000 (PubMed).

OReilly, Boehm, Shing et al.: "Endostatin: an endogenous inhibitor of angiogenesis and tumor growth." in: Cell, Vol. 88, Issue 2, pp. 277-85, 1997 (PubMed).

Rehn, Pihlajaniemi: "Alpha 1(XVIII), a collagen chain with frequent interruptions in the collagenous sequence, a distinct tissue distribution, and homology with type XV collagen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 10, pp. 4234-8, 1994 (PubMed).

Catalog No. ABIN108513
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