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Fetuin A antibody (alpha-2-HS-Glycoprotein)

Details for Product anti-AHSG Antibody No. ABIN108516, Supplier: Login to see
Antigen
  • ahs
  • a2hs
  • hsga
  • fetua
  • wu:fb63g02
  • zgc:103687
  • AHSG
  • MGC116429
  • A2HS
  • AHS
  • FETUA
  • HSGA
  • Aa2-066
  • pp63
Reactivity
Human
150
29
24
7
1
Host
Rabbit
106
23
23
20
2
1
Clonality
Polyclonal
Conjugate
This Fetuin A antibody is un-conjugated
13
11
8
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
126
102
41
23
19
14
11
11
11
8
5
2
2
1
1
1
1
1
Supplier
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Immunogen
  • The antibody was raised in rabbits by immunization with the Human Fetuin.
  • Source of Immunogen: Human serum
No Cross-Reactivity Human
Cross-Reactivity (Details) Not yet tested in other species.
Purification Immunoaffinity chromatography on a column with immobilized purified Human Fetuin.
Alternative Name Fetuin A (AHSG Antibody Abstract)
Background
  • Fetuin-A (AHSG), a 59 kDa glycoprotein, consisting of two cystatin-like domains and a smaller unrelated domain, is predominantly synthesized in liver. It is secreted into the blood stream and deposited as a noncollagenous protein in mineralized bones and teeth. Fetuin-A occurs in high serum concentrations during foetal life, whereas its level declines following infection, inflamatory and malignancy. Fetuin-A acts as an important circulating inhibitor of ectopic calcification, frequent complication of many degenerative diseases. Low serum level of Fetuin-A is associated with vascular and valvular calcification, atherosclerosis, malnutrition and higher cardiovascular mortality in chronic renal failure, liver cancer and liver cirrhosis patients on long-term dialysis. AHSG protein represents a natural inhibitor of tyrosinase kinase activity of the insulin receptor. It may play a significant role in regulating postprandial glucose disposal, insulin sensitivity, weight gain, and fat accumulation. The serum and bone-resident Fetuin-A binds to transforming growth factor-beta and blocks TGF-beta binding to cell surface receptors.
Pathways
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Supplier Images
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) image for anti-Fetuin A antibody (alpha-2-HS-Glycoprotein) (ABIN108516) Immunohistochemical staining of formalin-fixed paraffin-embedded human liver using th...
Background publications Ketteler, Gross, Ritz: "Calcification and cardiovascular problems in renal failure." in: Kidney international. Supplement, Issue 94, pp. S120-7, 2005 (PubMed).

Gangneux C, Daveau M, Hiron M et al.: "The inflammation-induced down-regulation of plasma Fetuin-A (alpha2HS-Glycoprotein) in liver results from the loss of interaction between long C/EBP isoforms at two neighbouring binding sites." in: Nucleic Acids Res, Vol. 31, Issue 20, pp. 5957-70, 2003 (PubMed).

Schafer, Heiss, Schwarz et al.: "The serum protein alpha 2-Heremans-Schmid glycoprotein/fetuin-A is a systemically acting inhibitor of ectopic calcification." in: The Journal of clinical investigation, Vol. 112, Issue 3, pp. 357-66, 2003 (PubMed).

Fernley RT, Wright RD, Coghlan JP: "Radioimmunoassay of carbonic anhydrase VI in saliva and sheep tissues." in: Biochem J, Vol. 274 ( Pt 2), pp. 313-6, 1991 (PubMed).