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Leptin (LEP) antibody

Antigen

Leptin (LEP)
Synonyms OB, OBS, FLJ94114, ob, obese
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
5 references available
Certificates ISO 9001:2008, ISO 13485:2003
Catalog no. ABIN108520
Quantity 0.1 mg  (Variants)
Price Product not available in this region.
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Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name Leptin
Format Affinity-purified
Description Specification: Anti-Human Leptin Antibody was raised in rabbits against recombinant human leptin.

Application Details
Publications Alternatives Variants back to top

Application Notes Titre: Is defined by indirect ELISA, it is >1/100,000 for antibody concentration 1mg/ml, 25 ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve.
Storage Store lyophilized antibody at 2-8°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time, it does not show decline in activity after two weeks at 4°C.
Research Area Hormones
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Maffei, Halaas, Ravussin et al.: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995 (PubMed).

Lonnqvist F, Arner P, Nordfors L et al.: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nat Med, Vol. 1, Issue 9, pp. 950-3, 1995 (PubMed).

Halaas, Gajiwala, Maffei et al.: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995 (PubMed).

Zhang, Proenca, Maffei et al.: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994 (PubMed).

Considine, Sinha, Heiman et al.: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Leptin (LEP)", type "Antibodies"
Hosts Rabbit (161), Mouse (43), Goat (12), Chicken (4), Sheep (3)
Reactivities Human (128), Mouse (Murine) (105), Rat (Rattus) (88), Pig (Porcine) (38), Cow (Bovine) (21), Sheep (Ovine) (20), Dog (Canine) (19), Horse (Equine) (18), Cat (Feline) (2), Chicken (1), Chimpanzee (1), Macaque (1)
Applications Western Blotting (WB) (144), ELISA (129), Immunofluorescence (IF) (51), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (22), Immunohistochemistry (IHC) (17), Immunoprecipitation (IP) (17), Immunohistochemistry (Frozen Sections) (IHC (fro)) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunocytochemistry (ICC) (8), Radioimmunoassay (RIA) (7), Enzyme Immunoassay (EIA) (6), Blocking Antibody (Inhibition) (4), ELISA (Detection) (3), Immunoelectron Microscopy (IEM) (3), ELISA (Capture) (2), Flow Cytometry (FACS) (2), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (2), Blocking Antibody (Inhibition) (1), Cellular Assay (CA) (1), Dot Blot (Dot) (1), Immunoassay (IA) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunostaining (ISt) (1), Neutralization (Neut) (1), Transfected cell culture (TCC) (1)
Conjugates Biotin (27), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), HRP (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3)
Epitopes AA 1-24 (3), C-Term (2), N-Term (2), N-Term, AA 1-24 (2), AA 1-42 (1), AA 1-48 (1), AA 149-167 (1), AA 25-44 (1), AA 48-167 (1), AA 57-70 (1), AA 92-145, cyclized (1), Internal Region (1), mouse (1), rat (1)