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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Leptin (LEP) antibody
|Synonyms||OB, OBS, FLJ94114, ob, obese|
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
|5 references available|
|Certificates||ISO 9001:2008, ISO 13485:2003|
|Quantity||0.1 mg (Variants)|
|Price||Product not available in this region.|
|Description||Specification: Anti-Human Leptin Antibody was raised in rabbits against recombinant human leptin.|
|Application Notes||Titre: Is defined by indirect ELISA, it is >1/100,000 for antibody concentration 1mg/ml, 25 ng of antigen are coated per well, and is then defined at a point of maximal decrease of the titration curve.|
|Storage||Store lyophilized antibody at 2-8°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80°C. Reconstituted antibody can be stored at 4°C for a limited period of time, it does not show decline in activity after two weeks at 4°C.|
|Restrictions||For Research Use only|
Maffei, Halaas, Ravussin et al.: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995 (PubMed).
Lonnqvist F, Arner P, Nordfors L et al.: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nat Med, Vol. 1, Issue 9, pp. 950-3, 1995 (PubMed).
Halaas, Gajiwala, Maffei et al.: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995 (PubMed).
Zhang, Proenca, Maffei et al.: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994 (PubMed).
Considine, Sinha, Heiman et al.: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996 (PubMed).