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Leptin antibody (LEP)

Details for Product anti-LEP Antibody No. ABIN108521, Supplier: Log in to see
Antigen
  • LEPD
  • OB
  • ob
  • obese
  • OBS
Alternatives
anti-Mouse (Murine) Leptin antibody for ELISA
Reactivity
Mouse (Murine), Rat (Rattus)
447
152
132
18
3
2
2
1
Host
Rabbit
350
247
20
8
4
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
66
25
19
13
9
9
7
6
6
6
6
6
6
6
6
6
6
6
6
5
5
3
3
3
3
3
3
2
2
Application
ELISA, Western Blotting (WB)
448
415
72
59
41
33
26
25
19
17
16
14
10
7
5
3
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Immunogen
  • The antibody was raised in rabbits by immunization with the recombinant Mouse Leptin. The immunization antigen (16 kDa) is a protein containing 147 amino acid residues of recombinant Mouse Leptin.
  • Source of Immunogen: E.coli
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Not yet tested in other species.
Purification Immunoaffinity chromatography on a column with immobilized recombinant Mouse Leptin.
Alternative Name Leptin (LEP Antibody Abstract)
Background
  • Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.
  • Other Names: Obesity factor, Obese protein, LEP, OB, OBS
  • Research Areas: Animal studies, Energy metabolism and body weight regulation, Reproduction
Research Area Cardiovascular, Atherosclerosis, Hormones
Pathways JAK-STAT Signaling, AMPK Signaling
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Background publications Halaas, Gajiwala, Maffei, Cohen, Chait, Rabinowitz, Lallone, Burley, Friedman: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995 (PubMed).

Maffei, Halaas, Ravussin, Pratley, Lee, Zhang, Fei, Kim, Lallone, Ranganathan: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995 (PubMed).

Lönnqvist, Arner, Nordfors, Schalling: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995 (PubMed).

Zhang, Proenca, Maffei, Barone, Leopold, Friedman: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994 (PubMed).