anti-Leptin antibody (LEP)

Details for Product anti-LEP Antibody No. ABIN108521
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Antigen
Synonyms ob, obese, LEPD, OB, OBS
Reactivity
Mouse (Murine), Rat (Rattus)
(332), (143), (125), (15), (3), (2), (2), (1)
Host
Rabbit
(321), (148), (21), (10), (4)
Clonality
Polyclonal
Conjugate
This Leptin antibody is un-conjugated
(56), (17), (13), (8), (8), (6), (3), (3), (3), (3), (3), (3), (3), (3)
Application
ELISA, Western Blotting (WB)
(351), (334), (68), (41), (36), (30), (24), (24), (22), (22), (22), (14), (10), (9), (8), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2)
Pubmed 4 references available
Quantity 0.1 mg
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Immunogen
  • The antibody was raised in rabbits by immunization with the recombinant Mouse Leptin. The immunization antigen (16 kDa) is a protein containing 147 amino acid residues of recombinant Mouse Leptin.
  • Source of Immunogen: E.coli
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) Not yet tested in other species.
Purification Immunoaffinity chromatography on a column with immobilized recombinant Mouse Leptin.
Alternative Name Leptin (LEP Antibody Abstract)
Background
  • Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa proteohormone consisting of 146 amino acid residues. Leptin is produced by differentiated adiocytes, although production have been demonstrated in other tissues, such as fundus of the stomach, the sceletal muscle, the liver, and the placenta. Leptin is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, in particular the hypothalamus, suppressing food intake and stimulating energy expenditure. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned.
  • Other Names: Obesity factor, Obese protein, LEP, OB, OBS
  • Research Areas: Animal studies, Energy metabolism and body weight regulation, Reproduction
Research Area Cardiovascular, Atherosclerosis, Hormones
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
General Zhang, Proenca, Maffei et al.: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994 (PubMed).

Halaas, Gajiwala, Maffei et al.: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995 (PubMed).

Lönnqvist, Arner, Nordfors et al.: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995 (PubMed).

Maffei, Halaas, Ravussin et al.: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995 (PubMed).

Validation Images
Catalog No. ABIN108521
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