Myostatin (MSTN) antibody

Details for Product No. ABIN108525
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Synonyms GDF-8, GDF8, gdf8, mstn, zgc:109749, Gdf8, NV13143, MSLHP, Cmpt
(157), (36), (24), (8), (2)
(125), (46), (10), (5), (5), (1), (1)
(19), (12), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB)
(117), (108), (53), (21), (16), (10), (10), (8), (3), (3), (2), (1)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States ( )
  • The antibody was raised in bovine by immunization with the recombinant Human Myostatin.
  • Source of Immunogen: E. coli
Sequence The immunization antigen (16.7 kDa) is a protein containing 152 AA of recombinant Human Myostatin. N-terminal His-tag including the spacer 43 AA. The AA sequence of the human myostatin part of the fusion protein is correspond ing to the UniProtKB/Swiss-Prot entry O14793, AA267-375. MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDPSSRSAVR SRRDFGLDCD EHSTESRCCR YPLTVDFEAF GWDWIIAPKR YKANYCSGEC EFVFLQKYPH THLVHQANPR GSAGPCCTPT KMSPINMLYF NGKEQIIYGK IPAMVVDRCG CS The amino acid sequence of the recombinant Human Myostatin is 100% homologous to the amino acid sequence of the Human Myostatin.
No Cross-Reactivity Human
Cross-Reactivity (Details) Not yet tested in other species.
Characteristics Source of Antigen: E. coli
Purification Immunoaffinity chromatography on a column with immobilized recombinant Human Myostatin.
Alternative Name Myostatin (MSTN Antibody Abstract)
  • Myostatin (GDF 8) is expressed uniquely in human skeletal muscle as a 12 kDa mature glycoprotein consisting of 109 amino acid residues and secreted into plasma. Myostatin is a member of the transforming growth factor beta superfamily of secreted growth and differentiation factors that is essential for proper regulation of skeletal muscle mass. Studies have shown that myostatin could play an important role in cardiac development and physiology. In serum, myostatin circulates as part of a latent complex containing myostatin propeptide and/or follistatin-related gene. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70 % ) of myostatin in serum bound to its propeptide. The myostatin propeptide is negative regulator of myostatin in vivo.
Research Area Hormones
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Background publications Sharma M, Kambadur R, Matthews KG et al.: "Myostatin, a transforming growth factor-beta superfamily member, is expressed in heart muscle and is upregulated in cardiomyocytes after infarct." in: J Cell Physiol, Vol. 180, Issue 1, pp. 1-9, 1999 (PubMed).

Gonzalez-Cadavid NF, Taylor WE, Yarasheski K et al.: "Organization of the human myostatin gene and expression in healthy men and HIV-infected men with muscle wasting." in: Proc Natl Acad Sci U S A, Vol. 95, Issue 25, pp. 14938-43, 1998 (PubMed).

McPherron AC, Lee SJ: "Double muscling in cattle due to mutations in the myostatin gene." in: Proc Natl Acad Sci U S A, Vol. 94, Issue 23, pp. 12457-61, 1997 (PubMed).

Catalog No. ABIN108525
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