sRANK (Soluble), (full length) antibody

Details for Product No. ABIN108528, Supplier: Log in to see
Soluble, full length
Western Blotting (WB)
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  • The antibody was raised in rabbits by immunization with the recombinant Human RANK.
  • Source of Immunogen: E. coli
Sequence The immunization antigen (19.3 kDa) is a protein containing 175 amino acid residues, comprising of the full-length of the TNF receptor-like extracellular domain of RANK receptor.
No Cross-Reactivity Human
Cross-Reactivity (Details) Not yet tested in other species.
Purification Immunoaffinity chromatography on a column with immobilized recombinant Human RANK.
  • The receptor activator of NF-kappaB (RANK), a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK(-/-) mice lack osteoclasts and have a profound defect in bone resorption and remodeling and in the development of the cartilaginous growth plates of endochondral bone. RANKL augments the ability of dendritic cells to stimulate naive T-cell proliferation in a mixed lymphocyte reaction, and increases the survival of RANK+ T cells generated with interleukin-4 and transforming growth factor (TGF)-beta. Thus RANK and RANKL seem to be important regulators of interactions between T cells and dendritic cells.
  • Other Names: soluble RANK Receptor
  • Research Areas: Bone and cartilage metabolism
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
Background publications Li J,Sarosi I,Yan XQ,Morony S,Capparelli C,Tan HL,McCabe S,Elliott R,Scully S,Van G,Kaufman S,Juan SC,Sun Y,Tarpley J,Martin L,Christensen K,McCabe J,Kostenuik P,Hsu H,Fletcher F,Dunstan CR,Lacey DL,Boyle WJ: "RANK is the intrinsic hematopoietic cell surface receptor that controls osteoclastogenesis and regulation of bone mass and calcium metabolism." in: Proc Natl Acad Sci U S A, Vol. 97, Issue 4, pp. 1566-71, 2000 (PubMed).

Nakagawa, Kinosaki, Yamaguchi, Shima, Yasuda, Yano, Morinaga, Higashio: "RANK is the essential signaling receptor for osteoclast differentiation factor in osteoclastogenesis." in: Biochemical and biophysical research communications, Vol. 253, Issue 2, pp. 395-400, 1999 (PubMed).

Prochownik EV,Kirsten WH: "Nucleic acid sequences of primate type C viruses in normal and neoplastic human tissues." in: Nature, Vol. 267, Issue 5607, pp. 175-7, 1977 (PubMed).

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