anti-Resistin antibody (RETN)

Details for Product anti-RETN Antibody No. ABIN108530
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Antigen
Synonyms RETN, ADSF, FIZZ3, RETN1, RSTN, XCP1, Fizz3, Rstn, Xcp4
Reactivity
Human
(204), (97), (57), (9), (2), (1)
Host
Rabbit
(213), (53), (48), (8), (8), (6)
Clonality
Polyclonal
Conjugate
This Resistin antibody is un-conjugated
(44), (16), (12), (8), (8), (8), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(239), (225), (58), (32), (27), (20), (14), (13), (12), (12), (7), (6), (4), (4), (4), (2), (2), (2), (1), (1)
Pubmed 8 references available
Quantity 0.1 mg
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Immunogen
  • The antibody was raised in rabbits by immunization with the recombinant Human Resistin.
  • Source of Immunogen: E. coli
No Cross-Reactivity Human
Cross-Reactivity (Details) Not yet tested in other species.
Purification Immunoaffinity chromatography on a column with immobilized recombinant Human Resistin.
Alternative Name Resistin (RETN Antibody Abstract)
Background
  • Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which is termed the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone). Human resistin contains 108 amino acids as a prepeptide, and its hydrofobic signal peptide is cleaved before its secretion. Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulace glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. Recent studies have shown that human resistin is expressed also in macrophages and may be a novel link between inflammation and insulin resistance.
Research Area Cardiovascular, Atherosclerosis, Metabolism, Hormones
Application Notes
  • Quality control: Indirect ELISA - to determine titer of the antibody SDS PAGE - to determine purity of the antibody
  • Otimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 0.1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation
Buffer The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Preservative Azide free
Storage 4 °C/-20 °C/-80 °C
Storage Comment The lyophilized antibody remains stable and fully active until the expiry date when stored at -20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after one week at 4 °C.
Expiry Date See label
General Hartman, Hu, Tyler et al.: "Mechanisms regulating adipocyte expression of resistin." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19754-61, 2002 (PubMed).

Steppan, Lazar: "Resistin and obesity-associated insulin resistance." in: Trends in endocrinology and metabolism: TEM, Vol. 13, Issue 1, pp. 18-23, 2001 (PubMed).

Fasshauer M, Klein J, Neumann S et al.: "Tumor necrosis factor alpha is a negative regulator of resistin gene expression and secretion in 3T3-L1 adipocytes." in: Biochem Biophys Res Commun, Vol. 288, Issue 4, pp. 1027-31, 2001 (PubMed).

Way, Görgün, Tong et al.: "Adipose tissue resistin expression is severely suppressed in obesity and stimulated by peroxisome proliferator-activated receptor gamma agonists." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25651-3, 2001 (PubMed).

Banerjee, Lazar: "Dimerization of resistin and resistin-like molecules is determined by a single cysteine." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 25970-3, 2001 (PubMed).

Kim, Lee, Moon et al.: "A cysteine-rich adipose tissue-specific secretory factor inhibits adipocyte differentiation." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 11252-6, 2001 (PubMed).

Steppan, Brown, Wright et al.: "A family of tissue-specific resistin-like molecules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 2, pp. 502-6, 2001 (PubMed).

Steppan, Bailey, Bhat et al.: "The hormone resistin links obesity to diabetes." in: Nature, Vol. 409, Issue 6818, pp. 307-12, 2001 (PubMed).

Validation Images
Catalog No. ABIN108530
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