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Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody

Details for Product No. ABIN108725, Supplier: Log in to see
Antigen
  • 2010110O04Rik
  • bb-1
  • BGP
  • Bgp
  • BGP1
  • Bgp1
  • BGPI
  • BGPR
  • C-CAM
  • Cc1
  • Ccam1
  • CD66a
  • Cea-1
  • Cea-7
  • Cea1
  • Cea7
  • CEACAM1
  • CEACAM8
  • hm:zeh0068
  • Hv-2
  • Hv2
  • mCEA1
  • MGC142545
  • Mhv-1
  • MHVR
  • MHVR1
  • mmCGM1
  • mmCGM1a
  • mmCGM2
  • zeh0068
Reactivity
Human
121
60
18
7
1
1
Host
Mouse
100
71
14
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
15
11
9
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Cell-ELISA (cELISA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
105
87
86
71
61
32
31
31
27
15
7
2
2
2
2
2
Supplier
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Immunogen genetic immunisation with cDNA encoding the extracellular region of human CEACAM1-A2
Clone GM-8G5
Isotype IgG1
Specificity GM8G5 recognises CEACAM1 transiently expressed on the cell surface of transfected BOSC cells as well as the naturally expressed protein (Fig. 2, kindly provided by Dr. R. Riesenberg, Labor für Tumorimmunologie, Urologische Klinik, Klinikum Grossh
Purification Protein G
Alternative Name CEACAM1 (CEACAM1 Antibody Abstract)
Background CEACAM1 (BGP) belongs to the carcinoembryonic antigen (CEA) gene family. CEACAM1 expression is downregulated in many tumors indicating a tumor-suppressive function. GM8G5 was generated by genetic immunisation and can be used to distinguish CEACAM1 from all other CEACAMs.
UniProt P13688
Application Notes Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
ELISA: 1:200 - 1:400
Immunohistology: 10 µg/mL (on cryosections)
For each application a titration should be performed to determine the optimal concentration.
Comment

Synonyms: CD66a, BGP, biliary glycoprotein I

Restrictions For Research Use only
Buffer PBS, pH 7.2
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C
Storage Comment short term: 2 °C - 8 °C, long term: -20 °C
Supplier Images
 image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody (ABIN108725) Specificity testing of GM8G5. BOSC cells were transiently transfected with expression...
 image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody (ABIN108725) GM8G5 on renal cell carcinoma (RCC). Hybridoma supernatant of GM8G5 recognises CEACAM...
 image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody (ABIN108725) Immunohistological staining of colon tissue with GM8G5. Cryosections of colon tissue ...
Flow Cytometry (FACS) image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody (ABIN108725) FACS analysis of BOSC23 cells using G5. BOSC23 cells were transiently trans-fected wi...
Flow Cytometry (FACS) image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody (ABIN108725) Members of the CEA family were expressed on BOSC cells after transient transfection w...
Immunohistochemistry (IHC) image for anti-Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (Biliary Glycoprotein) (CEACAM1) antibody (ABIN108725) Immunohistochemical staining on cryosections of colon tissue using G5. PBS containing...
Background publications Sémiramoth, Gleizes, Turbica, Sandré, Gorges, Kansau, Servin, Chollet-Martin: "Escherichia coli type 1 pili trigger late IL-8 production by neutrophil-like differentiated PLB-985 cells through a Src family kinase- and MAPK-dependent mechanism." in: Journal of leukocyte biology, Vol. 85, Issue 2, pp. 310-21, 2009 (PubMed).

Yue, Goldstein, Hollingsworth, Kaul, Brand, Haab: "The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays." in: Molecular & cellular proteomics : MCP, Vol. 8, Issue 7, pp. 1697-707, 2009 (PubMed).

Thomas, Zhu, Schnaar, Alves, Konstantopoulos: "Carcinoembryonic antigen and CD44 variant isoforms cooperate to mediate colon carcinoma cell adhesion to E- and L-selectin in shear flow." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15647-55, 2008 (PubMed).

Béaslas, Torreilles, Casellas, Simon, Fabre, Lacasa, Delers, Chambaz, Rousset, Carrière: "Transcriptome response of enterocytes to dietary lipids: impact on cell architecture, signaling, and metabolism genes." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 295, Issue 5, pp. G942-52, 2008 (PubMed).

Nittka, Böhm, Zentgraf, Neumaier: "The CEACAM1-mediated apoptosis pathway is activated by CEA and triggers dual cleavage of CEACAM1." in: Oncogene, Vol. 27, Issue 26, pp. 3721-8, 2008 (PubMed).

Yoon, Terada, Kita: "CD66b regulates adhesion and activation of human eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 12, pp. 8454-62, 2007 (PubMed).

Beau, Berger, Servin: "Rotavirus impairs the biosynthesis of brush-border-associated dipeptidyl peptidase IV in human enterocyte-like Caco-2/TC7 cells." in: Cellular microbiology, Vol. 9, Issue 3, pp. 779-89, 2007 (PubMed).

Avadhanula, Rodriguez, Devincenzo, Wang, Webby, Ulett, Adderson: "Respiratory viruses augment the adhesion of bacterial pathogens to respiratory epithelium in a viral species- and cell type-dependent manner." in: Journal of virology, Vol. 80, Issue 4, pp. 1629-36, 2006 (PubMed).

Tilki, Irmak, Oliveira-Ferrer, Hauschild, Miethe, Atakaya, Hammerer, Friedrich, Schuch, Galalae, Stief, Kilic, Huland, Ergun: "CEA-related cell adhesion molecule-1 is involved in angiogenic switch in prostate cancer." in: Oncogene, Vol. 25, Issue 36, pp. 4965-74, 2006 (PubMed).

Kammerer, Riesenberg, Weiler, Lohrmann, Schleypen, Zimmermann: "The tumour suppressor gene CEACAM1 is completely but reversibly downregulated in renal cell carcinoma." in: The Journal of pathology, Vol. 204, Issue 3, pp. 258-67, 2004 (PubMed).

Hammarström: "The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues." in: Seminars in cancer biology, Vol. 9, Issue 2, pp. 67-81, 1999 (PubMed).

Luo, Tapolsky, Earley, Wood, Wilson, Logothetis, Lin: "Tumor-suppressive activity of CD66a in prostate cancer." in: Cancer gene therapy, Vol. 6, Issue 4, pp. 313-21, 1999 (PubMed).