Pregnancy Specific beta-1-Glycoprotein 1 (PSG1) antibody

Antigen: Pregnancy Specific beta-1-Glycoprotein 1 (PSG1)

Synonyms: SP1, B1G1, PBG1, CD66f, PSBG1, PSGGA, DHFRP2, PSGIIA, FLJ90598, FLJ90654, PSG1

Clonality: Monoclonal (BAP3)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Catalog no.: ABIN108731

Additional Information

Alternative name: PSG1 (pregnancy-specific glycoprotein 1, CD66f)

Immunogen: BAC2 was generated by immunisation of BALB/c mice with a CEACAM7/Fc fusion protein (2). The antibody was purified by protein G affinity chromatography from cell culture supernatants and is routinely tested by flow cytometry on BOSC23 cells transiently tr

Format: Purified

Isotype: IgG1

Clone: BAP3

Description: BAP3 reacts with human pregnancy-specific glycoproteins (PSG), a group of closely related secreted glycoproteins which are highly expressed in placental syncytiotrophoblast cells of fetal origin (2). PSG are commonly expressed in tumors of trophoblast origin (hydatidiform mole, choriocarcinoma). They represent the most abundant fetal protein in the maternal blood at term. The eleven human PSG genes (PSG1-PSG11) form together with the carcinoembryonic antigen (CEA)-related cell adhesion molecule (CEACAM) genes the CEACAM gene family (1). PSG stimulate secretion of TH2-type cytokines from monocytes. CD9 was shown to represent the monocyte receptor for murine PSG17 (5). PSG are thought to modulate the maternal immune system during pregnancy thus protecting the semiallotypic fetus from rejection (3).

Specificity: BAC2 recognizes exclusively CEACAM7 transiently expressed on the cell surface of transfected BOSC23 cells (Fig. 1). BAC2 can be used to distinguish CEACAM7 from all other CEACAM and probably all pregnancy-specific glycoprotein molecules, namely CEACAM1 (B

Application Details

Application Notes: BAC2 has been tested for the use in flow cytometry (1.2 ug/10^6 cells) and cell-ELISA (1:200 t0 1:400)

Concentration: 100 ul of protein G affinity purified IgG, 1.0 mg/ml in phos-phate buffered saline, pH 7.2, 0.01% sodium azide

Storage: Store the antibody at 4°C

Restrictions: For Research Use only

Images

Detection of recombinant transmembrane-anchored PSG1 fusion protein on HeLa transfectants by BAP3. A HeLa transfectant expressing a recombinant, transmembrane-anchored form of PSG1 (red curve) and the corresponding parental cell line (green curve) were r

Publications

Sokolov, Kozliaeva, Mesniankina et al.: "[Isolation and purification of specific beta1-G-globulin]" in: Voprosy medit?sinsko? khimii, Vol. 24, Issue 2, pp. 240-4, 1978 (PubMed).

Zhou, Baranov, Zimmermann et al.: "Highly specific monoclonal antibody demonstrates that pregnancy-specific glycoprotein (PSG) is limited to syncytiotrophoblast in human early and term placenta." in: Placenta, Vol. 18, Issue 7, pp. 491-501, 1997 (PubMed).

Hammarstroem: "The carcinoembryonic antigen (CEA) family: structures, suggested functions and expression in normal and malignant tissues." in: Seminars in cancer biology, Vol. 9, Issue 2, pp. 67-81, 1999 (PubMed).

Snyder, Wessner, Wessells et al.: "Pregnancy-specific glycoproteins function as immunomodulators by inducing secretion of IL-10, IL-6 and TGF-beta1 by human monocytes." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 45, Issue 4, pp. 205-16, 2001 (PubMed).

Beauchemin, Draber, Dveksler et al.: "Redefined nomenclature for members of the carcinoembryonic antigen family." in: Experimental cell research, Vol. 252, Issue 2, pp. 243-9, 2001 (PubMed).

Waterhouse, Ha, Dveksler: "Murine CD9 is the receptor for pregnancy-specific glycoprotein 17." in: The Journal of experimental medicine, Vol. 195, Issue 2, pp. 277-82, 2002 (PubMed).