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Hyaluronan Synthase 2 (HAS2) (AA 67-170) antibody

Details for Product No. ABIN1098124, Supplier: Log in to see
Antigen
  • DG42
  • XHas2
  • Has2
Epitope
AA 67-170
14
11
7
3
2
Reactivity
Human
26
15
1
Host
Mouse
34
4
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
5
5
5
2
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
30
28
18
16
3
3
3
1
1
Supplier
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Immunogen Purified recombinant fragment of human HAS2 (AA 67-170) expressed in E. coli.
Clone 4-00E-07
Isotype IgG1
Purification Purified
Alternative Name HAS2 (HAS2 Antibody Abstract)
Background Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals, and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane, and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions, including space filling, lubrication of joints, and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition, the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes, and overexpression of HA receptors has been correlated with tumor metastasis. HAS2 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases, and its amino acid sequence shows significant homology to glycosaminoglycan synthetase (DG42) from Xenopus laevis, and human and murine hyaluronan synthase 1.
Molecular Weight 63.5 kDa
Gene ID 3037
HGNC 3037
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/100 - 1/500
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
ELISA image for anti-Hyaluronan Synthase 2 (HAS2) (AA 67-170) antibody (ABIN1098124) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Immunocytochemistry (ICC) image for anti-Hyaluronan Synthase 2 (HAS2) (AA 67-170) antibody (ABIN1098124) Figure 2: Immunofluorescence analysis of HeLa cells using HAS2 mouse mAb (green) . Bl...
Immunohistochemistry (IHC) image for anti-Hyaluronan Synthase 2 (HAS2) (AA 67-170) antibody (ABIN1098124) Figure 3: Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues us...
Western Blotting (WB) image for anti-Hyaluronan Synthase 2 (HAS2) (AA 67-170) antibody (ABIN1098124) Figure 4: Western blot analysis using HAS2 mouse mAb against NTERA-2 (1), HEK293 (2) ...
Western Blotting (WB) image for anti-Hyaluronan Synthase 2 (HAS2) (AA 67-170) antibody (ABIN1098124) Figure 5: Western blot analysis using HAS2 mAb against human HAS2 recombinant protein...
Product cited in: Okuda, Kobayashi, Xia et al.: "Hyaluronan synthase HAS2 promotes tumor progression in bone by stimulating the interaction of breast cancer stem-like cells with macrophages and stromal cells." in: Cancer research, Vol. 72, Issue 2, pp. 537-47, 2012 (PubMed).

Jokela, Makkonen, Oikari et al.: "Cellular content of UDP-N-acetylhexosamines controls hyaluronan synthase 2 expression and correlates with O-linked N-acetylglucosamine modification of transcription factors YY1 and SP1." in: The Journal of biological chemistry, Vol. 286, Issue 38, pp. 33632-40, 2011 (PubMed).